Celecoxib, a COX-2 inhibitor, synergistically potentiates the anti-inflammatory activity of docosahexaenoic acid in macrophage cell line
| Autor: | Akhilender Naidu Kamatham, Vijay Kondreddy |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Lipopolysaccharides
0301 basic medicine Docosahexaenoic Acids Immunology Nitric Oxide Synthase Type II Pharmacology Nitric Oxide Toxicology Dinoprostone Cell Line Nitric oxide Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Animals Immunology and Allergy Inflammation Cyclooxygenase 2 Inhibitors biology Interleukin-6 Tumor Necrosis Factor-alpha Macrophages Interleukin Drug Synergism General Medicine Eicosapentaenoic acid Nitric oxide synthase 030104 developmental biology chemistry Celecoxib Cyclooxygenase 2 Docosahexaenoic acid 030220 oncology & carcinogenesis biology.protein COX-2 inhibitor lipids (amino acids peptides and proteins) Tumor necrosis factor alpha medicine.drug |
| Zdroj: | Immunopharmacology and Immunotoxicology. 38:153-161 |
| ISSN: | 1532-2513 0892-3973 |
| Popis: | The anti-inflammatory properties of eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) and non-steroidal anti-inflammatory drugs overlap in many ways. The aim of this study was to examine the individual and synergetic anti-inflammatory effects of celecoxib, EPA and DHA in RAW-264.7 cell line.The cells were exposed to EPA, DHA, celecoxib, rosiglitazone, GW9662 alone or their combination, and stimulated with 5 μg/mL lipopolysaccharide (LPS). Nitric oxide (NO), tumor necrosis factor-α (TNF-α), interleukin (IL)-6 and prostaglandin-E2 (PGE2) levels were estimated in the medium using enzyme-linked immunosorbent assays. The cyclooxygenase-2 (COX-2) and inducible Nitric Oxide Synthase (iNOS) expression were analyzed in the cell lysate by immunoblotting. Peroxisome proliferator-activated receptor γ (PPARγ) and nuclear factor-κB (NF-κB) transcription factor activation assays were performed in the nuclear extract.Combined treatment of DHA (50 μM) and celecoxib (20 μM) significantly inhibited LPS induced synthesis of NO, TNF-α, IL-6 and PGE2 levels in the cells, compared to the individual treatments. In addition, DHA and celecoxib diminished the COX-2 and iNOS expression in the cells. This was associated with increased PPARγ activity, supressed NF-κB activity in the nucleus. We determined whether GW9662, a specific PPARγ inhibitor, could abolish the anti-inflammatory effect of DHA and celecoxib. GW9662 has abolished the DHA and celecoxib induced PPARγ activation, but did not alter the NF-κB mediated anti-inflammatory effects induced by celecoxib and DHA. Interestingly, EPA did not exhibit any inhibitory effect on these parameters.Our results suggest that DHA and celecoxib exhibit anti-inflammatory effect through inhibition of NF-κB, independent of PPARγ. Co-administration of celecoxib and DHA would be promising approach for the treatment of inflammatory diseases. |
| Databáze: | OpenAIRE |
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