Secreted frizzled related protein 1 protects H9C2 cells from hypoxia/re-oxygenation injury by blocking the Wnt signaling pathway
| Autor: | Hua-yin Li, Mayila Abudoukelimu, Yi-tong Ma, Chun-hui He, Fen Liu, Yi-Ning Yang, Xiao-Mei Li, Bang-Dang Chen, Jing Tao |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Frizzled Beta-catenin Cell Survival Endocrinology Diabetes and Metabolism Clinical Biochemistry Apoptosis Myocardial Reperfusion Injury Hypoxia reoxygenation injury Cell Line Wnt signaling pathway 03 medical and health sciences Endocrinology Bcl-2-associated X protein Secreted frizzled-related protein 1 medicine Animals Sfrp1 beta Catenin bcl-2-Associated X Protein Biochemistry medical biology Myocardium Research Biochemistry (medical) Membrane Proteins LRP6 LRP5 medicine.disease Cell Hypoxia Rats Cell biology 030104 developmental biology Gene Expression Regulation biology.protein Intercellular Signaling Peptides and Proteins H9C2 Reperfusion injury |
| Zdroj: | Lipids in Health and Disease |
| ISSN: | 1476-511X |
| DOI: | 10.1186/s12944-016-0240-5 |
| Popis: | Background In animal models, secreted frizzled related protein 1 (Sfrp1) inhibition of the Wnt signaling pathway is beneficial because Sfrp1 reduces myocardial apoptosis and prevents heart failure. The mechanisms mediating the cellular survival effect of Sfrp1 has not been completely elucidated. The present study was designed to investigate the possible protective actions of Sfrp1 on cardiac muscle cells using an in vitro model of ischemia/reperfusion, and to evaluate the possible involvement of the Wnt signaling pathway. Methods We used a recombinant AAV9 vector to deliver the Sfrp1 gene into H9C2 rat cardiomyoblasts and adopted an in vitro model of ischemia/reperfusion. Cell vitality was measured by CKK-8 and the trypan blue exclusion assay. Western blot was used to evaluate the expression of Dvl-1, β-catenin, c-Myc, Bax, and Bcl-2. Flow cytometry analysis of cardiomyocyte apoptosis was performed. Results We confirmed that Sfrp1 significantly increased cell viability (assayed by trypan blue and CKK-8) and decreased apoptosis (assayed by flow cytometry analysis and the Bax/Bcl-2 ratio). These effects were partly attributable to the ability of Sfrp1 to down-regulate Wnt signaling pathway (assayed by Western blot to evaluate the expression of Dvl-1, β-catenin, and c-Myc). Indeed, reactivation of the Wnt signaling pathway activity with the specific activator, Licl, reduced Sfrp1-induced cardioprotection during hypoxia and reoxygenation. Conclusions The present study demonstrated that Sfrp1 directly protected H9C2 cells from hypoxia and reoxygenation-induced reperfusion injury and apoptosis through inhibition of the Wnt signaling pathway, and added new mechanistic insight regarding the cardioprotective role of Sfrp1 on ischemic damage. Electronic supplementary material The online version of this article (doi:10.1186/s12944-016-0240-5) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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