In vitro fibrinolytic activity of an enzyme purified from Bacillus amyloliquefaciens strain KJ10 isolated from soybean paste
| Autor: | Gurupatham Devadhasan Biji, M.A. Rathi, Natarajan Benit, Jayarajapazham Rajaselvam, Srisesharam Srigopalram, Saqer S. Alotaibi, Ponnuswamy Vijayaraghavan |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Thrombolytic activity Lysis Bacillus amyloliquefaciens QH301-705.5 medicine.medical_treatment 01 natural sciences 03 medical and health sciences Column chromatography medicine Yeast extract Biology (General) chemistry.chemical_classification Chromatography Protease biology In vitro clot lysis Chemistry Cardiovascular therapeutics biology.organism_classification Enzyme assay Fibrinolytic protease 030104 developmental biology Enzyme biology.protein Fermentation Original Article General Agricultural and Biological Sciences 010606 plant biology & botany |
| Zdroj: | Saudi Journal of Biological Sciences, Vol 28, Iss 8, Pp 4117-4123 (2021) Saudi Journal of Biological Sciences |
| Popis: | A fibrinolytic protease secreting producing Bacillus amyloliquefaciens strain KJ10 was initially screened from the fermented soybean. Maximum productivity was obtained in the culture medium after 40 h incubation, 34 °C incubation temperature at pH 8.0. Fibrinolytic protease production was enhanced in the culture medium with 1% sucrose (3712 ± 52 U/mL), 1% (w/v) yeast extract (3940 ± 28 U/mL) and 0.1% MgSO4 (3687 ± 38 U/mL). Enzyme was purified up to 22.9-fold with 26%recovery after Q-Sepharose HP column chromatography. After three steps purification, enzyme activity was 1606U/mg and SDS-PAGE analysis revealed 29 kDa protein and enzyme band was detected by zymograpy. Enzyme was highly active at pH 8.0, at wide temperature ranges (40 °C − 55 °C) and was activated by Mn2+ (102 ± 3.1%) and Mg2+ (101.4 ± 2.9%) ions. The purified fibrinolytic enzyme was highly specific against N-Suc-Ala-Ala-Pro-Phe-pNA (189 mmol/min/mL) and clot lytic activity reached 28 ± 1.8% within 60 minin vitro. The purified fibrinolytic enzyme showed least erythrocytic lysis activity confirmed safety to prevent various health risks, including hemolytic anemia. Based on this study, administration of fibrinolytic enzyme from B. amyloliquefaciens strain KJ10 is safe for clinical applications. |
| Databáze: | OpenAIRE |
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