Assessment and prevention of cartilage degeneration surrounding a focal chondral defect in the porcine model
| Autor: | Andrew A. Tomaschke, Stephanie J. Bryant, Sarah A. Schoonraad, Elizabeth A. Aisenbrey, Mark A. Randolph, Joseph A. Wahlquist, Kristine M. Fischenich, Virginia L. Ferguson |
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| Rok vydání: | 2019 |
| Předmět: |
Cartilage
Articular 0301 basic medicine Swine medicine.medical_treatment Biophysics Osteoarthritis Degeneration (medical) Biochemistry Article Glycosaminoglycan 03 medical and health sciences 0302 clinical medicine medicine Animals Molecular Biology Cartilage degeneration Reduction (orthopedic surgery) Chemistry Cartilage Hydrogels Cell Biology medicine.disease Biomechanical Phenomena Disease Models Animal 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Self-healing hydrogels Female Proteoglycans Swelling medicine.symptom Cartilage Diseases Biomedical engineering |
| Zdroj: | Biochem Biophys Res Commun |
| ISSN: | 0006-291X |
| Popis: | Focal defects in articular cartilage are unable to self-repair and, if left untreated, are a leading risk factor for osteoarthritis. This study examined cartilage degeneration surrounding a defect and then assessed whether infilling the defect prevents degeneration. We created a focal chondral defect in porcine osteochondral explants and cultured them ex vivo with and without dynamic compressive loading to decouple the role of loading. When compared to a defect in a porcine knee four weeks post-injury, this model captured loss in sulfated glycosaminoglycans (sGAGs) along the defect's edge that was observed in vivo, but this loss was not load dependent. Loading, however, reduced the indentation modulus of the surrounding cartilage. After infilling with in situ polymerized hydrogels that were soft (100 kPa) or stiff (1 MPa) and which produced swelling pressures of 13 and 310 kPa, respectively, sGAG loss was reduced. This reduction correlated with increased hydrogel stiffness and swelling pressure, but was not affected by loading. This ex vivo model recapitulates sGAG loss surrounding a defect and, when infilled with a mechanically supportive hydrogel, degeneration is minimized. |
| Databáze: | OpenAIRE |
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