FoxO is required for the activation of hypertrehalosemic hormone expression in cockroaches
| Autor: | Marc Abrisqueta, José L. Maestro, Songül Süren-Castillo |
|---|---|
| Přispěvatelé: | Ministerio de Ciencia e Innovación (España), European Commission, Consejo Superior de Investigaciones Científicas (España) |
| Rok vydání: | 2014 |
| Předmět: |
endocrine system
Glycogenolysis animal structures Biophysics Cockroaches Biology Biochemistry Glucagon Glycogen phosphorylase chemistry.chemical_compound Transcription (biology) Hemolymph Animals Hypertrehalosemic/adipokinetic hormone Insulin RNA Small Interfering Molecular Biology Transcription factor Triglycerides Gene knockdown Glycogen Neuropeptides fungi Catabolism Forkhead Transcription Factors Insulin receptor Blattella germanica chemistry Adipose Tissue Starvation embryonic structures biology.protein Female FoxO hormones hormone substitutes and hormone antagonists |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | [Background] FoxO proteins are a subgroup of the Forkhead-box family of transcription factors, which function as the main transcriptional effectors of the insulin receptor pathway. This pathway, activated by the binding of insulin or IGFs (or insect insulin-like peptides), promotes the phosphorylation and inactivation of FoxO because of its export from the nucleus to the cytoplasm. The homolog of FoxO in the cockroach Blattella germanica works in a situation of nutrient shortage by inhibiting the endocrine induction of reproduction. [Methods] Using Blattella germanica as a model, we studied the functions of FoxO using RNA interference methodologies. We analyzed the mRNA levels of hypertrehalosemic hormone (HTH) and genes related to lipolysis, glycogenolysis and gluconeogenesis and quantified triacylglycerides, glycogen and trehalose. [Results] FoxO knockdown eliminates the starvation-induced expression of HTH in the corpora cardiaca. In addition, FoxO knockdown prevents the activation of the expression of Brummer lipase, glycogen phosphorylase and phosphoenolpyruvate carboxylase in the fat body of starved females. [Conclusions] Starvation-induced activation of FoxO stimulates the transcription of different genes related to catabolic processes, including HTH and genes involved in lipolysis, glycogenolysis and gluconeogenesis. [General significance] Our results show conservation in the action of the transcription factor FoxO in the activation of catabolic processes from basal insects to vertebrates. The results also describe a new and essentially different mode of action of transcription factor FoxO, which works through the activation of neuropeptide HTH expression, which will subsequently produce its own catabolic stimulatory function. © 2013 Elsevier B.V. This work was supported by grants BFU2006-01090/BFI (Spanish Ministry of Science and Innovation (MICINN) and FEDER) and BFU2010-15906 (MICINN) to J.L.M. M.A. and S. S.-C. are recipients of a pre-doctoral fellowship (MICINN) and a post-doctoral contract (CSIC, JAE program co-funded by the European Social Fund), respectively. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|