Microscale purification in support of high-throughput medicinal chemistry
| Autor: | Cullen L. Cavallaro, Merrill L. Davies, E. Scott Priestley, Fukang Yang, Chandan L. Barhate, Yong Zhang, Harold N. Weller, G. Greg Zipp, Andrew F. Donnell, Regina Black, Yingru Zhang, Ling Li |
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| Rok vydání: | 2021 |
| Předmět: |
Miniaturization
Material consumption business.industry Computer science Metals and Alloys General Chemistry Catalysis Surfaces Coatings and Films Electronic Optical and Magnetic Materials High-Throughput Screening Assays Small Molecule Libraries Workflow Tandem Mass Spectrometry Materials Chemistry Ceramics and Composites Process engineering business Throughput (business) Microscale chemistry Chromatography High Pressure Liquid Delay time |
| Zdroj: | Chemical communications (Cambridge, England). 57(84) |
| ISSN: | 1364-548X |
| Popis: | In recent years, successful assay miniaturization has enabled the exploration of synthesis scale reduction in pharmaceutical discovery. Miniaturization of pharmaceutical synthesis and purification allows a reduction in material consumption and shortens timelines, which ultimately reduces the cost per experiment without compromising data quality. Isolating and purifying the compounds of interest is a key step in the library synthesis process. In this manuscript we describe a high-throughput purification workflow in support of microscale (1–5 μmol or 0.5–2 mg) library synthesis. The optimized microscale purification system can routinely purify 384-well reaction plates with an analysis time of 4 min per sample. Instrument optimization, critical parameters such as column loading, delay time calibration, ultrafast pre- and post-purification analysis and library purification examples are provided. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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