Antiadenoviral Effect of the α5β1 Integrin Receptor Ligand, GRGDSP Peptide, in Serotypes That Cause Acute Keratoconjunctivitis
| Autor: | Kazuaki Kadonosono, Hiroaki Ishiko, Ryoji Kimura, Aya Fuchigami, Eiichi Uchio, Koki Aoki, Akio Hayashi, Shigeaki Ohno, Yuh-Huey Huang |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Serotype
Adenoviridae Infections Integrin Keratoconjunctivitis Peptide Biology Ligands Virus Replication medicine.disease_cause Antiviral Agents Virus Adenoviridae Cell Line medicine Humans Serotyping Receptor chemistry.chemical_classification Dose-Response Relationship Drug General Medicine Ligand (biochemistry) medicine.disease Virology Sensory Systems Ophthalmology chemistry Acute Disease biology.protein Oligopeptides Integrin alpha5beta1 |
| Zdroj: | Ophthalmologica. 221:326-330 |
| ISSN: | 1423-0267 0030-3755 |
| Popis: | Background/Aims: In adenoviral conjunctivitis, the infection process starts by the attachment of adenoviral fibers to conjunctival epithelial cells that contain the receptor for the adenovirus. The α5β1 integrin receptor ligand, GRGDSP peptide, contains the arginine-glycine-aspartate-binding motif which is common to the Coxsackie adenovirus receptor and integrins that are known to be adenoviral receptors. We evaluated the antiadenoviral effect of an expected adenoviral receptor inhibitor, GRGDSP peptide,in vitro. Methods: Adenovirus types 3, 4, 8, 19 and 37 were used. After calculating the 50% cytotoxic concentration of GRGDSP peptide, the adenovirus was cultivated with the agent for 7 days under serial dilution. Adenoviral DNA was qualitatively measured by real-time PCR. Results: GRGDSP peptide showed an inhibitory effect against adenoviral proliferation in all serotypes except type 4 in a dose-dependent manner. Conclusion: This result suggests that the α5β1 integrin receptor ligand, GRGDSP peptide, has antiadenoviral activity in vitro, and the possibility of being used for local treatment of epidemic keratoconjunctivitis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|