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Flow cytometry is an essential analytical technique used in biomedical diagnostics to measure properties of cells, micro-organisms, and particles. Laser light is scattered from particles focused in a flow cell and collected by light sensors, where the intensity of the scattered light is a function of the scattering angle, the refractive index of the particle and surrounding medium, the wavelength of light, and the size and the shape of the particle. One of the critical parts of the cytometer is the flow cell where the particle stream is constrained into a tight region within 10-30 μm using hydrodynamic focusing. The conventional flow cells use thick quartz flow cells, which are expensive and therefore not suitable for instruments targeted for resource-constrained settings. We demonstrate a compact, economical, bio-compatible flow cell assembly design that incorporates inexpensive and easily available capillaries attached to sturdy polymer fixtures in a simple manner that performs the focusing of a sample stream of particles. The flow cell has been tested by studying the relation between sample core diameter, and sample and sheath flow rates. Small-angle scattering (forward scatter) and wide-angle scattering (side scatter) have been captured for the enumeration and characterization of particles. We show excellent agreement between the size distribution obtained via direct imaging and that obtained from light scattering. The flow cell was also used to successfully size white blood cells in human blood samples. |
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