Temperature dependence of plasmodium falciparum erythrocytic stage development
| Autor: | Weerapong Phumratanaprapin, Kesinee Chotivanich, Parinya Kunawut, Arjen M. Dondorp, Yutatirat Singhaboot, Srisuda Keayarsa, Nattaporn Piaraksa |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
biology
Chemistry 030231 tropical medicine Plasmodium falciparum Hypothermia biology.organism_classification In vitro Andrology 03 medical and health sciences 0302 clinical medicine Infectious Diseases Cerebral Malaria Cytoplasm Virology medicine Parasite hosting Parasitology Antipyretic medicine.symptom Incubation medicine.drug |
| Popis: | Plasmodium falciparum infection causes febrile illness and severe disease with multiple organ failure and death when treatment is delayed. Antipyretic treatment is standard, and inducing hypothermia has been proposed to protect the brain in cerebral malaria. Here, we investigated the temperature dependence of asexual-stage parasite development and parasite multiplication in vitro. Plasmodium falciparum laboratory strain TM267 was incubated for 2 hours (short exposure) or 48 hours (continuous exposure) at different temperatures (32°C, 34°C, 35°C, 38°C, 39°C, and 40°C). The starting parasite developmental stage (ring, trophozoite, or schizont) varied between experiments. The parasite multiplication rate (PMR) was reduced under both hyper- and hypothermic conditions; after continuous exposure, the mean PMR ± SD was 9.1 ± 1.2 at 37°C compared with 2.4 ± 1.8 at 32°C, 2.3 ± 0.4 at 34°C, and 0.4 ± 0.1 at 40°C (P < 0.01). Changes in PMR were not significant after 2-hour exposure at temperatures ranging from 32°C to 40°C. Morphological changes in parasite cytoplasm and nucleus could be observed after long exposure to low or high temperature. After 48-hour incubation, rosette formation (≥ 2 uninfected red blood cells bound to infected red blood cells) was decreased at 34°C or 39°C compared with that at 37°C. In conclusion, both hyper- and hypothermia reduce PMR and delay erythrocytic stage development of P. falciparum, subsequently reducing rosette formation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|