Ribonucleic acid synthesis by spermatozoa from the rat and hamster
| Autor: | Charles Terner, Julia A. MacLaughlin |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
Male
Hamster Pronase In Vitro Techniques Biology Tritium Biochemistry Phosphates chemistry.chemical_compound Ribonucleases Nucleic Acids Cricetinae medicine Animals Ribonuclease Uridine Molecular Biology Deoxyribonucleases Hydrolysis RNA Deoxyribonuclease Cell Biology Epididymis Spermatozoa Molecular biology Rats Molecular Weight Glucose medicine.anatomical_structure chemistry Sephadex Chromatography Gel biology.protein Electrophoresis Polyacrylamide Gel Phosphorus Radioisotopes |
| Zdroj: | Biochemical Journal. 133:635-639 |
| ISSN: | 0264-6021 |
| DOI: | 10.1042/bj1330635 |
| Popis: | Spermatozoa from the cauda of the epididymis of the hamster and rat were incubated with [5-3H]uridine and glucose. By using a procedure avoiding bacterial and other cellular contamination, sonic extracts were prepared and digested with deoxyribonuclease and Pronase. Radioactive RNA of high molecular weight was isolated by two methods: (a) gel filtration on Sephadex G-75 columns and (b) polyacrylamide-gel electrophoresis in which it migrated in the region of 28S and 23S RNA markers. The macromolecules were alkali-labile and hydrolysed by ribonuclease. From 3H radioactivity and E260 of the isolated RNA the rate of incorporation of uridine into RNA of spermatozoa was calculated to be 0.1–0.5nmol/h per mg of RNA. |
| Databáze: | OpenAIRE |
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