An integrated approach for automating validation of extracted ion chromatographic peaks
| Autor: | William D. Nelson, Bert C. Lynn, Kert Viele |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Statistics and Probability
Proteomics Chromatography Proteome Computer science Quantitative proteomics Proteins Integrated approach Mass spectrometry Biochemistry Protein expression Mass Spectrometry Computer Science Applications Computational Mathematics Applications Note Computational Theory and Mathematics Isotope Labeling Databases Protein Molecular Biology Algorithms |
| Popis: | Summary: Accurate determination of extracted ion chromatographic peak areas in isotope-labeled quantitative proteomics is difficult to automate. Manual validation of identified peaks is typically required. We have integrated a peak confidence scoring algorithm into existing tools which are compatible with analysis pipelines based on the standards from the Institute for Systems Biology. This algorithm automatically excludes incorrectly identified peaks, improving the accuracy of the final protein expression ratio calculation. Contact: wnels2@uky.edu Source and Supplementary Information: http://www.chem.uky.edu/research/lynn/Nelson.pdf |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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