| Autor: |
Haojun Yuan, Wanlei Gao, Jiawen Yin, Kai Chen, Ying Mu, Qinghui Jin, Chunping Jia, Hui Cong, Jiancheng Yu, Jianlong Zhao |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Analytical biochemistry. 656 |
| ISSN: |
1096-0309 |
| Popis: |
The lack of reliable and practical method for detecting rare hot mutation of epidermal growth factor receptor (EGFR) in circulating tumor DNA (ctDNA) for lung cancer has remained a challenge for general clinical application due to excess wild type DNA in clinical samples. In this study, we developed a droplet digital PCR (ddPCR) platform, integrating a PDMS chip and double-layer glass reservoir. The duplex T-junction droplet generators in PDMS chip can produce about one million uniform droplets of 4.187 pL within ∼10 min, which were then stored in the glass reservoir. The double-layer glass reservoir can protect droplets from evaporation and breaking, solving the problem of instability during thermal-cycling. The quantitative capabilities of the ddPCR chip were evaluated by testing EGFR exon gene 21, with a good linear correlation in the wide range of 10 |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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