Microbiomic comparison of the intestine of the earthworm Eisenia fetida fed ergovaline
Autor: | Forrest Smith, A. Morrie Craig, Sudeep Perumbakkam, Rogan Rattray |
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Rok vydání: | 2009 |
Předmět: |
Eisenia fetida
Ergot Alkaloids Rumen Animal feed Microorganism Poaceae Applied Microbiology and Biotechnology Microbiology Ergovaline RNA Ribosomal 16S Botany Escherichia coli Animals Vasoconstrictor Agents Food science Horses Oligochaeta Sheep biology Bacteria business.industry Earthworm Genetic Variation General Medicine biology.organism_classification Animal Feed Immunity Innate Ergotamines RNA Bacterial Microbial population biology Neotyphodium Animals Domestic Livestock Cattle business Digestive System Plasmids |
Zdroj: | Current microbiology. 60(3) |
ISSN: | 1432-0991 |
Popis: | Tall fescue toxicosis and ergot alkaloids cost U.S. livestock producers approximately one billion dollars in annual livestock production loss annually. Ergovaline (EV) is the tall fescue alkaloid primarily responsible for clinical disease in livestock. Since native ruminal microorganisms have not been attributed to the detoxification of EV, finding detoxifying microbes from other environments is desirable. One possible source for potential microorganisms that can degrade EV is the anaerobic gut of the earthworm, Eisenia fetida. This study describes a comparative microbial analysis of earthworm digestive tracts receiving 10,000 ppb EV (E+ treatment) when compared with a control treatment with no detectable amounts of EV (E− treatment). An HPLC assay determined a 25% loss of EV from the E+ treatment was microbial in nature. A community microbiomic approach of constructing 16S-rRNA gene clone libraries was used to compare the microbes affected by the two treatments. RDPII tools such as Classifier and Libcompare were used in the analysis of 16S sequences. DOTUR analysis was used to examine the richness and diversity of the two microbial populations in these experiments. The results indicate there are few significant differences in the microbial community structure between the two microbiomes. |
Databáze: | OpenAIRE |
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