Exosomes mediate an epithelial‐mesenchymal transition cascade in retinal pigment epithelial cells: Implications for proliferative vitreoretinopathy

Autor: Hetian Lei, Hui Li, Yao Zhang, Haipei Yao, Jiabin Pan, Min Li, Haiying Jin, Kaizhe Wang, Shuai Yang, Fang Wang
Rok vydání: 2020
Předmět:
0301 basic medicine
Proliferative vitreoretinopathy
Epithelial-Mesenchymal Transition
retinal pigment epithelium
Biology
Exosomes
Benzylidene Compounds
Exosome
proliferative vitreoretinopathy
Cell Line
Transforming Growth Factor beta2
03 medical and health sciences
0302 clinical medicine
Cell Line
Tumor
microRNA
medicine
Humans
Epithelial–mesenchymal transition
Cell Proliferation
Gene Library
epithelial‐mesenchymal transition
Aniline Compounds
Retinal pigment epithelium
MicroRNA sequencing
Vitreoretinopathy
Proliferative
Epithelial Cells
Original Articles
Cell Biology
Transforming growth factor beta
medicine.disease
eye diseases
Microvesicles
Cell biology
MicroRNAs
030104 developmental biology
medicine.anatomical_structure
Gene Expression Regulation
030220 oncology & carcinogenesis
embryonic structures
biology.protein
Nanoparticles
Molecular Medicine
Original Article
sense organs
Zdroj: Journal of Cellular and Molecular Medicine
ISSN: 1582-4934
1582-1838
Popis: Exosomes have recently emerged as a pivotal mediator of many physiological and pathological processes. However, the role of exosomes in proliferative vitreoretinopathy (PVR) has not been reported. In this study, we aimed to investigate the role of exosomes in PVR. Transforming growth factor beta 2 (TGFß‐2) was used to induce epithelial‐mesenchymal transition (EMT) of retinal pigment epithelial (RPE) cells, as an in vitro model of PVR. Exosomes from normal and EMTed RPE cells were extracted and identified. We incubated extracted exosomes with recipient RPE cells, and co‐cultured EMTed RPE cells and recipient RPE cells in the presence of the exosome inhibitor GW4869. Both experiments suggested that there are further EMT‐promoting effects of exosomes from EMTed RPE cells. MicroRNA sequencing was also performed to identify the miRNA profiles in exosomes from both groups. We identified 34 differentially expressed exosomal miRNAs (P
Databáze: OpenAIRE
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