Immunochemical Studies of High and Low Molecular Forms of Urokinase
| Autor: | Kenji Akazawa, Tadayoshi Kosugi, Hiroyuki Sumi, Susumu Maehara, Naotika Toki, Hisashi Mihara, Osamu Matsuo |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Antigenicity
Protein Conformation Immunoglobulin light chain Antigen-Antibody Reactions Protein structure Endopeptidases medicine Animals Humans Binding site Urokinase Antiserum chemistry.chemical_classification Fibrin Binding Sites Chemistry Immune Sera food and beverages Hematology General Medicine Ouchterlony double immunodiffusion Urokinase-Type Plasminogen Activator Molecular biology Molecular Weight Enzyme Biochemistry Rabbits medicine.drug |
| Zdroj: | Acta Haematologica. 67:263-267 |
| ISSN: | 1421-9662 0001-5792 |
| Popis: | The immunochemical properties of high and low molecular forms of urokinase (HMW-UK, MW 53,000, 124,000 IU/mg protein; LMW-UK, MW 32,000, 230,000 IU/mg protein) were studied with specific antisera against the functionally active heavy chain (H chain, MW 31,000, 201,000 IU/mg protein) and the light chain (L chain, MW 18,000) of HMW-UK. Using a double immunodiffusion technique, LMW-UK did not demonstrate L chain antigenicity in the molecule. Anti-L-chain serum exerted no effect on LMW-UK and the H chain, but anti-H-chain serum strongly inhibited the fibrinolytic activity of all the active enzymes (HMW-UK, LMW-UK, and H chain). Anti-L chain serum was found to exert an antifibrinolytic effect on HMW-UK. |
| Databáze: | OpenAIRE |
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