The Efficiency of Arabidopsis thaliana Floral Dip Transformation Is Determined Not Only by the Agrobacterium Strain Used but Also by the Physiology and the Ecotype of the Dipped Plant
| Autor: | Anna Depicker, Sylvie De Buck, Jonah Nolf, Rim Ghedira |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
DNA
Bacterial Physiology Agrobacterium Arabidopsis Virulence Flowers Transformation Genetic Plasmid Species Specificity Botany Arabidopsis thaliana Ecotype Genetics biology Strain (chemistry) fungi Gene Transfer Techniques food and beverages General Medicine Plants Genetically Modified biology.organism_classification Transformation (genetics) Agronomy and Crop Science Plasmids |
| Zdroj: | Molecular Plant-Microbe Interactions®. 26:823-832 |
| ISSN: | 1943-7706 0894-0282 |
| DOI: | 10.1094/mpmi-11-12-0267-r |
| Popis: | To evaluate the chromosomal background of different Agrobacterium strains on floral dip transformation frequency, eight wild-type Agrobacterium strains, provided by Laboratorium voor Microbiologie Gent (LMG) and classified in different genomic groups, were compared with the commonly used Agrobacterium strains C58C1 Rifr (pMP90) and LBA4404 in Arabidopsis thaliana Columbia (Col-0) and C24 ecotypes. The C58C1 Rifr chromosomal background in combination with the pMP90 virulence plasmid showed high Col-0 floral dip transformation frequencies (0.76 to 1.57%). LMG201, which is genetically close to the Agrobacterium C58 strain, with the same virulence plasmid showed comparable or even higher transformation frequencies (1.22 to 2.28%), whereas the LBA4404 strain displayed reproducibly lower transformation frequencies (r (pMP90) and LBA4404 reference strains. None of the strains could transform the C24 ecotype with a frequency higher than 0.1%. Strikingly, all Arabidopsis Col-0 floral dip transformation experiments showed a high transformation variability from plant to plant (even more than 50-fold) within and across the performed biological repeats for all analyzed Agrobacterium strains. Therefore, the physiology of the plant and, probably, the availability of competent flowers to be transformed determine, to a large extent, floral dip transformation frequencies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|