Nd:YAG laser radiation (1.064 nm) accelerates differentiation of osteoblasts to osteocytes on smooth and rough titanium surfacesin vitro
| Autor: | Niklaus P. Lang, Costas Psarros, Ioannis A. Vrotsos, K. Kyriakidou, Ioannis K. Karoussis |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Materials science
Surface Properties Cellular differentiation Lasers Solid-State In Vitro Techniques Cell morphology law.invention 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine law Cell Adhesion medicine Irradiation Low-Level Light Therapy Cell adhesion Cells Cultured Cell Proliferation Titanium Osteoblasts Cell growth Cell Differentiation Osteoblast 030206 dentistry Laser medicine.anatomical_structure Nd:YAG laser Microscopy Electron Scanning Biophysics Oral Surgery |
| Zdroj: | Clinical Oral Implants Research. 28:785-790 |
| ISSN: | 0905-7161 |
| Popis: | Background A number of studies revealed beneficial effects of low-level laser therapy (LLLT) regarding cell proliferation and differentiation. Aim To investigate the effect of Nd:YAG (1.064 nm) laser radiation in the proliferation and differentiation potential of MG-63 osteoblast-like cells. Additionally, the effects of the surface configurations were to be evaluated. Material and methods MG-63 osteoblast cells were cultured on different surfaces: plastic tissue culture, smooth (polished) titanium-PT and rough titanium-SLA. The effects of both titanium surfaces and low-level laser therapy (LLLT) on cell adhesion were evaluated by the gene expression of molecules involved in cell proliferation and differentiation. In addition, scanning electron microscopy (SEM) and MTT proliferation assays were used to examine cell morphology and proliferation, respectively. Results Compared to smooth (PT) surfaces, SLA surfaces favoured MG-63 cell differentiation. Following the application of Nd:YAG laser irradiation, cells yielded statistically significantly improved differentiation on both smooth and SLA surfaces compared with non-irradiated surfaces. Conclusions The findings of this present study suggest that both surface morphology and Nd:YAG laser irradiation influence the proliferation and differentiation potential of MG-63 cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text