Induction of glyoxylate cycle enzymes in rat liver upon food starvation
| Autor: | Vasily N. Popov, Abir U. Igamberdiev, S. V. Volvenkin, Claus Schnarrenberger |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Isocitrate lyase
Rat liver Biophysics Glyoxylate cycle Biology Microbodies Biochemistry Structural Biology Malate synthase Organelle Centrifugation Density Gradient Genetics Animals Molecular Biology Ammonium sulfate precipitation Differential centrifugation chemistry.chemical_classification Glyoxylates Cell Biology Hydrogen-Ion Concentration Peroxisome Rats Kinetics Enzyme Liver chemistry Starvation Enzyme Induction biology.protein |
| Zdroj: | FEBS Letters. 390:258-260 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(96)00668-0 |
| Popis: | The key enzymes of the glyoxylate cycle, isocitrate lyase and malate synthase, have been detected in liver of food-starved rats. Activities became measurable 3 days and peaked 5 days after the beginning of starvation. Both enzymes were found in the peroxisomal cell fraction after organelle fractionation by isopycnic centrifugation. Isocitrate lyase was purified 112-fold by ammonium sulfate precipitation, and chromotography on DEAE-cellulose and Toyopearl HW-65. The specific activity of the purified enzyme was 9.0 units per mg protein. The K(m)(isocitrate) was 68 microM and the pH optimum was at pH 7.4. Malate synthase was enriched 4-fold by ammonium sulfate precipitation. The enzyme had a K(m)(acetyl-CoA) of 0.2 microM, a K(m)(glyoxylate) of 3 mM and a pH optimum of 7.6. |
| Databáze: | OpenAIRE |
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