Multilocus genotyping of Cryptosporidium and Giardia in non-outbreak related cases of diarrhoea in human patients in Belgium
| Autor: | Jozef Vercruysse, Edwin Claerebout, G De Groote, Aline Visser, Stijn Casaert, Simone M. Cacciò, Thomas Geurden, Bruno Levecke |
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| Rok vydání: | 2009 |
| Předmět: |
Diarrhea
Giardiasis Genotype Sequence analysis Protozoan Proteins Cryptosporidiosis Cryptosporidium Locus (genetics) Microbiology Apicomplexa Belgium Species Specificity Risk Factors parasitic diseases Prevalence Animals Humans biology Molecular epidemiology Giardia Outbreak Sequence Analysis DNA DNA Protozoan biology.organism_classification Virology Infectious Diseases Animal Science and Zoology Parasitology Triose-Phosphate Isomerase |
| Zdroj: | Parasitology. 136(10) |
| ISSN: | 1469-8161 |
| Popis: | SUMMARYStool samples from Belgian patients suffering from abdominal pain and/or diarrhoea were examined for Cryptosporidium and Giardia. Cryptosporidium-positive samples were genotyped using the 70 kDa heat shock protein and the 60 kDa glycoprotein (GP60) genes: C. hominis was identified in 54·2% and C. parvum in 45·8% of the samples. Sequencing at the GP60 locus indicated that subgenotype IbA10G2 of C. hominis and subgenotype IIaA15G2R1 of C. parvum were the most prevalent, although several other subgenotypes were identified. For Giardia, sequencing at the β-giardin, triose phosphate isomerase (TPI) and glutamate dehydrogenase (GDH) genes revealed assemblage B as the most prevalent (74·4%) in human patients. A high degree of heterogeneity was found, especially on the β-giardin gene, and to a lesser extent on the GDH gene. Furthermore, using a novel species-specific PCR based on the TPI gene, mixed infections with both assemblage A and B were detected in a large number (32·4%) of human patients, which might have important epidemiological implications. |
| Databáze: | OpenAIRE |
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