Dengue Virus Directly Stimulates Polyclonal B Cell Activation
| Autor: | Ana Carolina Egypto Rosa Berbel, Ana Theresa Silveira de Morais, Arturo Ramon Vargas Correa, Luciana Barros de Arruda, Ligia M.T. Peçanha, Michelle Premazzi Papa |
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| Rok vydání: | 2015 |
| Předmět: |
viruses
Lymphocyte Naive B cell B-cell receptor lcsh:Medicine Antibodies Viral Lymphocyte Activation Real-Time Polymerase Chain Reaction Immunoglobulin G Cell Line Tetraspanin 28 Dengue Aedes medicine Animals Humans lcsh:Science B cell B-Lymphocytes Multidisciplinary biology Interleukin-6 lcsh:R virus diseases HLA-DR Antigens Dengue Virus biochemical phenomena metabolism and nutrition Molecular biology B-1 cell Phenotype medicine.anatomical_structure Immunoglobulin M Immunoglobulin class switching biology.protein RNA Viral lcsh:Q B7-2 Antigen Mitogen-Activated Protein Kinases Antibody Research Article Signal Transduction |
| Zdroj: | PLoS ONE, Vol 10, Iss 12, p e0143391 (2015) PLoS ONE |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0143391 |
| Popis: | Dengue infection is associated to vigorous inflammatory response, to a high frequency of activated B cells, and to increased levels of circulating cross-reactive antibodies. We investigated whether direct infection of B cells would promote activation by culturing primary human B lymphocytes from healthy donors with DENV in vitro. B cells were susceptible, but poorly permissive to infection. Even though, primary B cells cultured with DENV induced substantial IgM secretion, which is a hallmark of polyclonal B cell activation. Notably, DENV induced the activation of B cells obtained from either DENV immune or DENV naïve donors, suggesting that it was not dependent on DENV-specific secondary/memory response. B cell stimulation was dependent on activation of MAPK and CD81. B cells cultured with DENV also secreted IL-6 and presented increased expression of CD86 and HLA-DR, which might contribute to B lymphocyte co-stimulatory function. Indeed, PBMCs, but not isolated B cells, secreted high amounts of IgG upon DENV culture, suggesting that interaction with other cell types in vivo might promote Ig isotype switching and IgG secretion from different B cell clones. These findings suggest that activation signaling pathways triggered by DENV interaction with non-specific receptors on B cells might contribute to the exacerbated response observed in dengue patients. |
| Databáze: | OpenAIRE |
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