Qualitative enzymatic detection of organophosphate and carbamate insecticides
| Autor: | Dilek Nartop, Elvan Hasanoğlu Özkan, Nurdan Kurnaz Yetim, Nurşen Sarı |
|---|---|
| Přispěvatelé: | [Belirlenecek] |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Insecticides
Carbamate Spectrophotometry Infrared Methiocarb Polymers organophosphate medicine.medical_treatment Methomyl 02 engineering and technology 010402 general chemistry 01 natural sciences polymers containing azomethine Gel permeation chromatography Carbofuran Immobilization chemistry.chemical_compound medicine Selectivity Pesticides Acephate Chromatography Organophosphate Spectrometry X-Ray Emission carbamate Organothiophosphorus Compounds General Medicine Enzymes Immobilized 021001 nanoscience & nanotechnology Condensation reaction Amides Pollution Organophosphates 0104 chemical sciences Biosensors chemistry Malathion Acetylcholinesterase Phosphoramides tris(2-aminoethyl)amine polymer-bound Carbamates Chlorpyrifos Cholinesterase Inhibitors 0210 nano-technology Stability Food Science |
| Popis: | In this study, new polymers containing amides (TrisPS-Ntaa, andTrisPS-Ntaa-Fc) were synthesized by condensation reaction for qualitative identification of insecticides. The synthesized polymers, including amides were investigated by infrared spectroscopy (IR), scanning electron microscopy-energy dispersion X- ray spectrometry (SEM-EDX), and gel permeation chromatography (GPC). Then, acetylcholinesterase enzyme (AChE) was covalently immobilized on these polymers to improve properties (including activity, reusability, and storage stability). Accordingly, organophosphate (malathion, acephate, chlorpyrifos methyl) and carbamate (carbofuran, methiocarb, methomyl), which are used to prevent harmful organisms in some agricultural products were enzymatically determined based on their inhibitory activity on AChE. Duzce University Research FundDuzce University [2017.07.06.661] This work was supported by Duzce University Research Fund (Project number: 2017.07.06.661). WOS:000551299400001 2-s2.0-85088478841 PubMed: 32698655 |
| Databáze: | OpenAIRE |
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