Vitamin C depletion increases superoxide generation in brains of SMP30/GNL knockout mice

Autor: Yoshitaka Kondo, Yasunori Sato, Akihito Ishigami, Kyu-Shik Jeong, Toru Sasaki, Setsuko Handa, Shingo Aizawa, Mizuki Iwama, Naoki Maruyama, Mitsugu Fukuda, Nobuko Shimada, Jaewon Lee, Masumi Akita, Akiko Amano
Rok vydání: 2008
Předmět:
Zdroj: Biochemical and biophysical research communications. 377(1)
ISSN: 1090-2104
Popis: Vitamin C (VC) has a strong antioxidant function evident as its ability to scavenge superoxide radicals in vitro. We verified that this property actually exists in vivo by using a real-time imaging system in which Lucigenin is the chemiluminescent probe for detecting superoxide in senescence marker protein-30 (SMP30)/gluconolactonase (GNL) knockout (KO) mice, which cannot synthesize VC in vivo. SMP30/GNL KO mice were given 1.5 g/L VC [VC(+)] for 2, 4, or 8 weeks or denied VC [VC(-)]. At 4 and 8 weeks, VC levels in brains from VC(-) KO mice were
Databáze: OpenAIRE