Vitamin C depletion increases superoxide generation in brains of SMP30/GNL knockout mice
| Autor: | Yoshitaka Kondo, Yasunori Sato, Akihito Ishigami, Kyu-Shik Jeong, Toru Sasaki, Setsuko Handa, Shingo Aizawa, Mizuki Iwama, Naoki Maruyama, Mitsugu Fukuda, Nobuko Shimada, Jaewon Lee, Masumi Akita, Akiko Amano |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Antioxidant
medicine.medical_treatment Biophysics Ascorbic Acid medicine.disease_cause Biochemistry Antioxidants chemistry.chemical_compound Mice In vivo Superoxides medicine Animals Lucigenin Molecular Biology Mice Knockout Luminescent Agents Superoxide Body Weight Calcium-Binding Proteins Intracellular Signaling Peptides and Proteins Brain Cell Biology Ascorbic acid Molecular biology chemistry Knockout mouse Gluconolactonase Acridines Carboxylic Ester Hydrolases Oxidative stress |
| Zdroj: | Biochemical and biophysical research communications. 377(1) |
| ISSN: | 1090-2104 |
| Popis: | Vitamin C (VC) has a strong antioxidant function evident as its ability to scavenge superoxide radicals in vitro. We verified that this property actually exists in vivo by using a real-time imaging system in which Lucigenin is the chemiluminescent probe for detecting superoxide in senescence marker protein-30 (SMP30)/gluconolactonase (GNL) knockout (KO) mice, which cannot synthesize VC in vivo. SMP30/GNL KO mice were given 1.5 g/L VC [VC(+)] for 2, 4, or 8 weeks or denied VC [VC(-)]. At 4 and 8 weeks, VC levels in brains from VC(-) KO mice were |
| Databáze: | OpenAIRE |
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