Tyrosine decarboxylase activity of Enterococcus mundtii: new insights into phenotypic and genetic aspects
| Autor: | Sandra Torriani, Chiara Montanari, Veronica Gatto, Fausto Gardini, Valentina Prodomi, Giulia Tabanelli, Eleonora Bargossi |
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| Přispěvatelé: | Gatto, Veronica, Tabanelli, Giulia, Montanari, Chiara, Prodomi, Valentina, Bargossi, Eleonora, Torriani, Sandra, Gardini, Fausto |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Enterococcus mundtii Sequence analysis 030106 microbiology tyrDC gene expression Sequence Homology Tyramine biogenic amines Bioengineering Biology Biochemistry Applied Microbiology and Biotechnology Evolution Molecular 03 medical and health sciences chemistry.chemical_compound Gene Order Phenethylamines Tyrosine Gene Research Articles Enterococcus mundtii biogenic amines tyramine 2-phenylethylamine tyrDC gene expression Permease Gene Expression Profiling 2-phenylethylamine Sequence Analysis DNA Tyrosine Decarboxylase biology.organism_classification DNA Fingerprinting Tyrosine decarboxylase Random Amplified Polymorphic DNA Technique Tyrosine decarboxylase activity chemistry Enterococcus Biotechnology Research Article |
| Zdroj: | Microbial Biotechnology |
| ISSN: | 1751-7915 |
| Popis: | Summary Few information is available about the tyraminogenic potential of the species Enterococcus mundtii. In this study, two plant‐derived strains of E. mundtii were selected and investigated to better understand the phenotypic behaviour and the genetic mechanisms involved in tyramine accumulation. Both the strains accumulated tyramine from the beginning of exponential phase of growth, independently on the addition of tyrosine to the medium. The strains accumulated also 2‐phenylethylamine, although with lower efficiency and in greater extent when tyrosine was not added. Accordingly, the tyrosine decarboxylase (tyrDC) gene expression level increased during the exponential phase with tyrosine added, while it remained constant and high without precursor. The genetic organization as well as sequence identity levels of tyrDC and tyrosine permease (tyrP) genes indicated a correlation with those of phylogenetically closer enterococcal species, such as E. faecium, E. hirae and E. durans; however, the gene Na+/H+ antiporter (nhaC) that usually follow tyrP is missing. In addition, BLAST analysis revealed the presence of additional genes encoding for decarboxylase and permease in the genome of several E. mundtii strains. It is speculated the occurrence of a duplication event and the acquisition of different specificity for these enzymes that deserves further investigations. |
| Databáze: | OpenAIRE |
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