LBL deposition of chitosan and silk fibroin on nanofibers for improving physical and biological performance of patches
| Autor: | Yanxiang Cheng, Fenghua Tao, Youmei Long, Liangbin Xia, Hongbing Deng, Dan Li, Yanqing Wang, Fangfang Dai, Li Huang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Staphylococcus aureus
Biocompatibility Cell Survival Nanofibers Fibroin Biocompatible Materials 02 engineering and technology Biochemistry Chitosan Mice 03 medical and health sciences chemistry.chemical_compound Pelvic floor dysfunction Structural Biology Elderly population Cell Adhesion Escherichia coli medicine Animals Molecular Biology Mechanical Phenomena 030304 developmental biology 0303 health sciences Pelvic floor reconstruction Water General Medicine Fibroblasts Hydrogen-Ion Concentration 021001 nanoscience & nanotechnology medicine.disease Anti-Bacterial Agents chemistry Nanofiber Fibroins 0210 nano-technology Hydrophobic and Hydrophilic Interactions Biomedical engineering |
| Zdroj: | International Journal of Biological Macromolecules. 130:348-356 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2019.02.147 |
| Popis: | Pelvic floor dysfunction diseases (PFD) become more prevalent with the increase of elderly population, and complications of pelvic floor reconstructive surgery (e.g. infection and exposure of mesh) have been troubling to patients and gynecologists. In this study, the nanofibrous mats were prepared by alternately depositing chitosan (CS) and silk fibroin (SF) on Nylon6 (N6) nanofibrous mats via layer-by-layer (LBL) technique. The as-prepared mats were characterized. The results showed that CS and SF molecules were successfully assembled on the nanofibers. Additionally, after LBL modification, the hydrophilicity of the nanofibrous mats was reduced and the mechanical properties were improved. Furthermore, the antibacterial activity of the LBL-structured mats reached >95% inhibiting Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). The in vitro cell co-culture experiments indicated that LBL-structured mats had smaller toxic effects and more excellent biocompatibility to L929 fibroblasts, especially the mats with 15 bilayers coated films. Hence, the LBL-structured mats are promising materials for pelvic floor reconstruction to reduce postoperative pelvic complication rates. |
| Databáze: | OpenAIRE |
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