Human granzyme B mediates cartilage proteoglycan degradation and is expressed at the invasive front of the synovium in rheumatoid arthritis
| Autor: | Ruud A. Bank, J. A. D. M. de Roos, Johan M. TeKoppele, F. C. Breedveld, Johan Hendrikus Verheijen, W.H. van der Laan, H.K. Ronday, Paul P. Tak, Christopher J. Froelich, Cornelis Erik Hack, Pancras C.W. Hogendoorn |
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| Přispěvatelé: | Other departments, Gaubius Instituut |
| Rok vydání: | 2001 |
| Předmět: |
collagen
rheumatoid arthritis Pathology Biomedical Research Arthritis sulfate Granzymes Arthritis Rheumatoid Metacarpophalangeal Joint Extracellular matrix granzyme B Biology Health hydroxyproline Pharmacology (medical) isotope labeling proline Cells Cultured biology tritium bone erosion Serine Endopeptidases Synovial Membrane cartilage degeneration cartilage matrix Extracellular Matrix medicine.anatomical_structure priority journal immunohistochemistry Proteoglycans joint destruction musculoskeletal diseases medicine.medical_specialty Cartilage destruction collagen degradation synovium Cartilage metabolism animal tissue protein denaturation Chondrocytes Rheumatology glycosaminoglycan medicine Animals Humans controlled study protein expression proteoglycan nonhuman business.industry human cell Cartilage explant proteoglycan synthesis medicine.disease Molecular biology sulfur 35 Granzyme B Granzyme Proteoglycan biology.protein Cattle Synovial membrane business |
| Zdroj: | Scopus-Elsevier Rheumatology, 1, 40, 55-61 Rheumatology (Oxford, England), 40(1), 55-61. Oxford University Press |
| ISSN: | 1462-0332 1462-0324 |
| DOI: | 10.1093/rheumatology/40.1.55 |
| Popis: | Objective. To investigate the cartilage-degrading capacity of granzyme B and the presence of granzyme B-positive cells at sites of erosion in the rheumatoid synovium. Methods. Granzyme B was added to [3H]proline/[35S]sulphate-labelled cartilage matrices and to cartilage explants. Proteoglycan degradation was assessed by the release of 35S and glycosaminoglycans into the medium and collagen degradation was assessed by the release of 3H and hydroxyproline and by measuring the fraction of denatured collagen. Granzyme B expression was studied at the invasive front of the synovium by immunohistochemistry. Results. Granzyme B induced loss of both newly synthesized, radiolabelled proteoglycans in cartilage matrices and resident proteoglycans of the cartilage explants. No effect on collagen degradation was found. Granzyme B-positive cells were present throughout the synovium and at the invasive front. Conclusion. The presence of granzyme B-positive cells at the invasive front of the synovium together with its ability to degrade articular proteoglycans supports the view that granzyme B may contribute to joint destruction in rheumatoid arthritis. |
| Databáze: | OpenAIRE |
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