A novel C1q domain containing protein in black rockfish (Sebastes schlegelii) serves as a pattern recognition receptor with immunoregulatory properties and possesses binding activity to heat-aggregated IgG
| Autor: | Qin-qin Gu, Xue Du, Jing-jing Wang, Min Zhang, Bin Yue, Guang-hua Wang, Yong-hua Hu, Shun Zhou |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Fish Proteins
0301 basic medicine Signal peptide Vibrio anguillarum ved/biology.organism_classification_rank.species Aquatic Science Fish Diseases Open Reading Frames 03 medical and health sciences Protein Domains Animals Humans Environmental Chemistry Amino Acid Sequence Peptide sequence Disease Resistance Vibrio Black rockfish Innate immune system biology ved/biology Complement C1q Pattern recognition receptor 04 agricultural and veterinary sciences General Medicine biology.organism_classification Perciformes 030104 developmental biology Biochemistry Immunoglobulin G Receptors Pattern Recognition Vibrio Infections 040102 fisheries C1q domain 0401 agriculture forestry and fisheries Sebastes schlegelii |
| Zdroj: | Fish & Shellfish Immunology. 87:73-81 |
| ISSN: | 1050-4648 |
| DOI: | 10.1016/j.fsi.2019.01.004 |
| Popis: | C1q-domain-containing (C1qDC) proteins, which are involved in a series of immune responses, are important pattern recognition receptors in innate immunity in vertebrates and invertebrates. Functional studies of C1qDC proteins in vertebrates are scarce. In the present study, a C1qDC protein (SsC1qDC) from the teleost black rockfish (Sebastes schlegelii) was identified and examined at expression and functional levels. The open reading frame of SsC1qDC is 636 bp, and the predicted amino acid sequence of SsC1qDC shares 62%-69% overall identity with the C1qDC proteins of several fish species. SsC1qDC possesses conserved C1qDC features, including a signal sequence and a C1q domain. SsC1qDC was expressed in different tissues and its expression was up-regulated by bacterial and viral infection. Recombinant SsC1qDC (rSsC1qDC) exhibited apparent binding activities against PAMPs including LPS and PGN. rSsC1qDC had antibacterial activity against Vibrio parahaemolyticus, and was able to enhance the phagocytic activity of macrophages towards Vibrio anguillarum. rSsC1qDC interacted with human heat-aggregated IgG. Furthermore, in the presence of rSsC1qDC, fish exhibited enhanced resistance against bacterial infection. Collectively, these results indicated that SsC1qDC serves as a pattern recognition receptor and plays a vital role in the defense system of black rockfish. |
| Databáze: | OpenAIRE |
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