Phosphate transporters, PnPht1;1 and PnPht1;2 from Panax notoginseng enhance phosphate and arsenate acquisition
| Autor: | Zhang Xue, Wen Gu, Jie Yu, Zhao Ronghua, He Sen, Xi-Fu Wang, Li Zedong, Cao Guanhua, Di Chen |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Mutant Saccharomyces cerevisiae Panax notoginseng Plant Science 010501 environmental sciences 01 natural sciences Phosphates chemistry.chemical_compound lcsh:Botany Phosphate Transport Proteins Arsenate exposure Amino Acid Sequence Phylogeny Plant Proteins 0105 earth and related environmental sciences chemistry.chemical_classification biology Arsenate Phosphate deficiency Transporter biology.organism_classification lcsh:QK1-989 Amino acid Phosphate transporter chemistry Biochemistry Symporter Arsenates Heterologous expression Sequence Alignment Research Article 010606 plant biology & botany |
| Zdroj: | BMC Plant Biology BMC Plant Biology, Vol 20, Iss 1, Pp 1-14 (2020) |
| ISSN: | 1471-2229 |
| DOI: | 10.1186/s12870-020-2316-7 |
| Popis: | Background Panax notoginseng is a medicinally important Chinese herb with a long history of cultivation and clinical application. The planting area is mainly distributed in Wenshan Prefecture, where the quality and safety of P. notoginseng have been threatened by high concentration of arsenic (As) from the soil. The roles of phosphate (Pi) transporters involved in Pi acquisition and arsenate (AsV) tolerance were still unclear in this species. Results In this study, two open reading frames (ORFs) of PnPht1;1 and PnPht1;2 separated from P. notoginseng were cloned based on RNA-seq, which encoded 527 and 541 amino acids, respectively. The results of relative expression levels showed that both genes responded to the Pi deficiency or As exposure, and were highly upregulated. Heterologous expression in Saccharomyces cerevisiae MB192 revealed that PnPht1;1 and PnPht1;2 performed optimally in complementing the yeast Pi-transport defect, particularly in PnPht1;2. Cells expressing PnPht1;2 had a stronger AsV tolerance than PnPht1;1-expressing cells, and accumulated less As in cells under a high-Pi concentration. Combining with the result of plasma membrane localization, these data confirmed that transporters PnPht1;1 and PnPht1;2 were putative high-affinity H+/H2PO4− symporters, mediating the uptake of Pi and AsV. Conclusion PnPht1;1 and PnPht1;2 encoded functional plasma membrane-localized transporter proteins that mediated a putative high-affinity Pi/H+ symport activity. Expression of PnPht1;1 or PnPht1;2 in mutant strains could enhance the uptake of Pi and AsV, that is probably responsible for the As accumulation in the roots of P. notoginseng. |
| Databáze: | OpenAIRE |
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