Retinoic acid improves maturation rate and upregulates the expression of antioxidant-related genes inin vitromatured buffalo (Bubalus bubalis) oocytes
| Autor: | Salah El-Din El-Assal, Ashraf M. El-Sayed, Ahmed Al Amin, Ahmed Gad, Swiefy A. Swiefy, Said Abu Hamed, M. A. E. Khalifa |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Vitamin lcsh:Veterinary medicine Antioxidant General Veterinary medicine.medical_treatment Metabolite Retinoic acid Biology medicine.disease_cause Oocyte In vitro Cell biology 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology medicine.anatomical_structure chemistry medicine lcsh:SF600-1100 Gene Oxidative stress |
| Zdroj: | International Journal of Veterinary Science and Medicine, Vol 6, Iss 2, Pp 279-285 (2018) |
| ISSN: | 2314-4599 |
| DOI: | 10.1016/j.ijvsm.2018.09.003 |
| Popis: | Retinoic acid, vitamin A metabolite, plays a role in oocyte development and maturation in different ways including gene expression alteration and/or prohibiting oxidative stress. The objective of this study was to examine the effect of 9-cis-retinoic acid (9-cisRA) on the quality and maturation rate of buffalo oocytes. Cumulus-oocyte complexes (COCs, n = 460) were collected from ovaries of slaughtered buffalos. Varying concentrations of 9-cisRA (0, 5, 50, and 200 nM) were added to the maturation medium, and the following parameters were analyzed: (i) maturation and cleavage rates, (ii) mitochondrial activity and reactive oxygen species (ROS) levels, (iii) expression level of antioxidant-related genes (PRDX1, SOD1, CAT, HOMX1, and GPX4) using RT-qPCR. Maturation rate was significantly improved in 5 nM 9-cisRA oocyte group (95.8%, P |
| Databáze: | OpenAIRE |
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