Improvement in inflammation and airway remodelling after acupuncture at BL13 and ST36 in a mouse model of chronic asthma
| Autor: | Ambar Mudigdo, Muthmainah Muthmainah, Diding Heri Prasetyo, Bambang Purwanto, Ida Nurwati, Koosnadi Saputra |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Acupuncture Therapy Inflammation Chronic inflammatory disease 03 medical and health sciences Mice 0302 clinical medicine Chronic asthma Acupuncture Medicine Animals Humans Airway Remodelling Lung Asthma Mice Inbred BALB C biology business.industry General Medicine medicine.disease Ovalbumin Disease Models Animal 030104 developmental biology Steroid therapy 030228 respiratory system Complementary and alternative medicine Immunology Chronic Disease biology.protein Airway Remodeling Female Neurology (clinical) medicine.symptom business Acupuncture Points |
| Zdroj: | Acupuncture in medicine : journal of the British Medical Acupuncture Society. 37(4) |
| ISSN: | 1759-9873 |
| Popis: | Background: Asthma is a chronic inflammatory disease that affects millions of people worldwide. Chronic asthma is commonly resistant to steroid therapy. Acupuncture has an anti-inflammatory effect and has been widely used as an add-on therapy for asthma. Objective: To evaluate the effects of acupuncture on the inflammatory response and airway remodelling in the bronchioles of an asthma mouse model. Methods: A chronic asthma model was produced in female BALB/c mice by ovalbumin (OVA) sensitisation. 32 mice were randomised into four groups: control; asthma (OVA); OVA+BL13; and OVA+BL13+ST36. OVA was administered by intraperitoneal injection on days 0 and 14 followed by aerosol exposure of 1% OVA three times a week for 6 weeks. Manual acupuncture (MA) was performed three times a week for 6 weeks at BL13 alone, or BL13 in combination with ST36, in the two MA-treated groups. At the end of the experiment, blood samples were collected to determine eosinophil and neutrophil counts and lung tissue was prepared for histological examination. Results: A pronounced reduction in the neutrophil count was achieved after MA at BL13+ST36 (P=0.005) while the eosinophil count was lowered after MA both at BL13 (P=0.007) and BL13+ST36 (P=0.006). Reduction in the bronchiolar epithelial and smooth muscle thickness and the number of goblet cells was observed after MA at BL13 (P=0.001, P=0.001 and P=0.002, respectively) and BL13+ST36 (P=0.001, P=0.002 and P=0.001, respectively). Conclusion: Acupuncture can reduce the inflammatory response and prevent airway remodelling in a chronic asthma mouse model. |
| Databáze: | OpenAIRE |
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