Plasmid profile and role in virulence of salmonella enterica serovars isolated from food animals and humans in Lagos Nigeria
| Autor: | Fowora Muinah, Ajayi Abraham, Bode-Sojobi Ibidunni Oreoluwa, Smith Stella Ifeanyi, Adeleye Isaac Adeyemi, Kalpy Julien Coulibaly |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Serotype Salmonella 030231 tropical medicine 030106 microbiology Nigeria Virulence Microbial Sensitivity Tests Biology medicine.disease_cause Microbiology Article 03 medical and health sciences 0302 clinical medicine Plasmid Antibiotic resistance medicine Animals Humans Salmonella Infections Animal Sheep Public Health Environmental and Occupational Health Salmonella enterica Hemolysin General Medicine biology.organism_classification Anti-Bacterial Agents Ciprofloxacin Infectious Diseases Salmonella Infections Cattle Parasitology Chickens Plasmids medicine.drug |
| Zdroj: | Pathog Glob Health |
| ISSN: | 2047-7732 2047-7724 |
| DOI: | 10.1080/20477724.2019.1691364 |
| Popis: | Infections caused by non-typhoidal Salmonella (NTS) are common around the world, with high morbidity and mortality rates recorded annually. Salmonella serovars harbor plasmids of various sizes which may play roles in antibiotic resistance and virulence. The aim of this study was to profile and determine the role of plasmids in ciprofloxacin resistance and virulence of Salmonella serovars. Using alkaline lysis method 25 NTS serovars from food animals and humans were assayed for plasmids. Isolates ability to resist healthy human serum, bind congo red, produce hemolysin and susceptibility to ciprofloxacin before and after plasmid curing were evaluated. Mobility of plasmids was determined by conjugation. Fifteen (60%) of the 25 Salmonella serovars harbored plasmids with sizes ranging from 0.4 to 38.4 kb. S. Budapest serovars harbored 5–9 plasmids, while S. Essen and S. Mura had six plasmids each. S. Chomedey and a S. Budapest serovar were sensitive to ciprofloxacin after plasmid curing while other serovars remained resistant to ciprofloxacin after plasmid curing. All Salmonella isolates had the ability to withstand human serum before and after plasmid curing, however, some serovars lost their ability to bind congo red after plasmid curing. All Salmonella isolates that initially displayed hemolysin activity retained their ability after curing. Thirteen (86.7%) of the 15 serovars that harbored plasmids conjugatively transferred their plasmids to E. coli K-12 (DH5α). Having Salmonella serovars that harbor transferrable plasmids in the food chain can drive antibiotic resistance and enhanced virulence of otherwise less virulence strains. |
| Databáze: | OpenAIRE |
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