Leptin Fully Suppresses Acetylcholine-Induced Insulin Secretion and is Reversed by Tolbutamide in Isolated Perfused Chicken Pancreas
| Autor: | Yacir Benomar, M. Derouet, S. Crochet, Mohammed Taouis, Nicole Rideau |
|---|---|
| Přispěvatelé: | Unité de Recherches Avicoles (URA), Institut National de la Recherche Agronomique (INRA), Laboratoire de Biologie Cellulaire et Moléculaire, Biotechnologies, INRA |
| Rok vydání: | 2003 |
| Předmět: |
Leptin
[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology Endocrinology Diabetes and Metabolism medicine.medical_treatment Clinical Biochemistry MESH: Diazoxide MESH: Base Sequence MESH: Insulin Secretion Biochemistry Cholinergic Antagonists MESH: Recombinant Proteins Endocrinology MESH: Reverse Transcriptase Polymerase Chain Reaction Insulin Secretion Insulin MESH: Animals MESH: Cholinergic Antagonists MESH: Receptors Cell Surface 0303 health sciences MESH: Kinetics [SDV.NEU.PC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior Reverse Transcriptase Polymerase Chain Reaction digestive oral and skin physiology MESH: Chickens [SDV.NEU.SC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences 04 agricultural and veterinary sciences General Medicine Recombinant Proteins Insulin oscillation Perfusion medicine.anatomical_structure Receptors Leptin Pancreas MESH: Perfusion medicine.drug MESH: DNA Primers medicine.medical_specialty animal structures Tolbutamide Receptors Cell Surface MESH: Receptors Leptin MESH: Insulin In Vitro Techniques Biology Islets of Langerhans 03 medical and health sciences Internal medicine medicine Diazoxide MESH: Tolbutamide Animals DNA Primers 030304 developmental biology MESH: In Vitro Techniques Leptin receptor Base Sequence MESH: Acetylcholine MESH: Islets of Langerhans Pancreatic islets Biochemistry (medical) 0402 animal and dairy science MESH: Leptin 040201 dairy & animal science Acetylcholine Kinetics Chickens |
| Zdroj: | Hormone and Metabolic Research Hormone and Metabolic Research, Thieme Publishing, 2003, 35 (2), pp.81-85. ⟨10.1055/s-2003-39055⟩ |
| ISSN: | 1439-4286 0018-5043 |
| Popis: | International audience; So far, there has been no evidence for any direct pancreatic effect of leptin in the chicken. The present study was aimed at detecting chicken leptin receptor (cOb-R) expression in isolated chicken islets of Langerhans and to examine the direct effect of leptin on insulin secretion after stimulation by acetylcholine (1 micro M) + glucose (14 mM) from isolated perfused chicken pancreas. We will show that i) full length cOb-R mRNA was expressed in isolated pancreatic islets of chickens, ii) recombinant chicken leptin (10 nM) or diazoxide (100 micro M) rapidly (within 2 min) and significantly suppressed insulin secretion induced by acetylcholine stimulation without any change in volume outflow rate, iii) tolbutamide (100 micro M) introduced 10 min after leptin and perfused for 10 min fully reversed the suppressive effect of leptin on pre-established acetylcholine-induced insulin release. In conclusion, we found that leptin has a profound inhibitory influence upon insulin secretion in perfused chicken pancreas. The results suggest that leptin inhibits insulin secretion by acting before or at the level of K ATP channels in chicken pancreatic beta-cells. Further studies are warranted to clarify the specific inhibitory mechanism. |
| Databáze: | OpenAIRE |
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