Plaque Formation by and Plaque Cloning of Chlamydia trachomatis Biovar Trachoma
| Autor: | Akira Matsumoto, Hiroshi Izutsu, Masanobu Ohuchi, Naoyuki Miyashita |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Microbiology (medical)
Chlamydiology and Rickettsiology Biovar Restriction Mapping Chlamydia trachomatis medicine.disease_cause Polymerase Chain Reaction Microbiology chemistry.chemical_compound Parrots Plasmid medicine Animals Humans Chlamydiaceae Cloning Molecular DNA Fungal Infectivity biology Glycogen biology.organism_classification medicine.disease Virology Kinetics Microscopy Electron Chlamydophila psittaci chemistry Trachoma Chlamydiales Chromosomes Fungal Plasmids |
| Zdroj: | Journal of Clinical Microbiology. 36:3013-3019 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.36.10.3013-3019.1998 |
| Popis: | A new technique for the induction of plaque formation by Chlamydia trachomatis biovar trachoma applicable to the titration of infectivity and cloning of biovar trachoma was established. Three novel strains were cloned and confirmed to be free of glycogen inclusions. The lack of glycogen accumulation correlated with the absence of a 7.5-kb plasmid, which is highly conserved in other strains of C. trachomatis . Although the growth efficiency of these plasmid-free strains was slightly lower than that of plasmid-positive strains, possession of the plasmid and glycogen accumulation were not essential for the survival of C. trachomatis . |
| Databáze: | OpenAIRE |
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