Cell Wall Modifications in Arabidopsis Plants with Altered α-l -Arabinofuranosidase Activity
| Autor: | Lynette M. Fulton, Frédérique Bitton, Mélanie Marquis, Christopher S. Cobbett, Philippe Ranocha, Lise Jouanin, Alain Jauneau, Jean-Pierre Renou, Ricardo A. Chávez Montes, Luc Saulnier, Deborah Goffner, Zoran Minic, Yves Martinez |
|---|---|
| Přispěvatelé: | Laboratoire de Recherche en Sciences Végétales (LRSV), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Surfaces Cellulaires et Signalisation chez les Végétaux (SCSV), Laboratoire de biologie cellulaire et moléculaire, Institut National de la Recherche Agronomique (INRA), Department of Chemistry, University of Saskatchewan [Saskatoon] (U of S), Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), University of Melbourne, Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM), Unité de recherche en génomique végétale (URGV), Institut National de la Recherche Agronomique (INRA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Dynamique et Evolution des Parois cellulaires végétales, Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3) |
| Rok vydání: | 2008 |
| Předmět: |
0106 biological sciences
STRUCTURE Physiology Mutant Arabidopsis PLANTE TRANSGENIQUE Plant Science IMMUNOLOGIE 01 natural sciences PAROI CELLULAIRE Arabidopsis thaliana ARAF1 CELL WALL Glucuronidase Oligonucleotide Array Sequence Analysis 0303 health sciences Plant Stems Monosaccharides food and beverages MONOSACCHARIDE Immunohistochemistry EXPRESSION DES GENES Cell biology Phenotype Xylosidases Biochemistry ACTIVITE ENZYMATIQUE ARABINOFURANOSIDASE Research Article DNA Bacterial GENE EXPRESSION ARABINANE Biology COMPOSITION CHIMIQUE [SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics Cell wall 03 medical and health sciences Polysaccharides ENZYMATIC ACTIVITY Parenchyma Genetics Cambium 030304 developmental biology Arabidopsis Proteins Gene Expression Profiling ARABINAN fungi Xylem biology.organism_classification GENETIC ENGINEERING Mutagenesis Insertional TRANSFORMATION GENETIQUE Phloem 010606 plant biology & botany |
| Zdroj: | Plant Physiology Plant Physiology, 2008, 147 (1), pp.63-77. ⟨10.1104/pp.107.110023⟩ Plant Physiology, American Society of Plant Biologists, 2008, 147 (1), pp.63-77. ⟨10.1104/pp.107.110023⟩ |
| ISSN: | 1532-2548 0032-0889 |
| Popis: | Although cell wall remodeling is an essential feature of plant growth and development, the underlying molecular mechanisms are poorly understood. This work describes the characterization of Arabidopsis (Arabidopsis thaliana) plants with altered expression of ARAF1, a bifunctional α-l-arabinofuranosidase/β-d-xylosidase (At3g10740) belonging to family 51 glycosyl-hydrolases. ARAF1 was localized in several cell types in the vascular system of roots and stems, including xylem vessels and parenchyma cells surrounding the vessels, the cambium, and the phloem. araf1 T-DNA insertional mutants showed no visible phenotype, whereas transgenic plants that overexpressed ARAF1 exhibited a delay in inflorescence emergence and altered stem architecture. Although global monosaccharide analysis indicated only slight differences in cell wall composition in both mutant and overexpressing lines, immunolocalization experiments using anti-arabinan (LM6) and anti-xylan (LM10) antibodies indicated cell type-specific alterations in cell wall structure. In araf1 mutants, an increase in LM6 signal intensity was observed in the phloem, cambium, and xylem parenchyma in stems and roots, largely coinciding with ARAF1 expression sites. The ectopic overexpression of ARAF1 resulted in an increase in LM10 labeling in the secondary walls of interfascicular fibers and xylem vessels. The combined ARAF1 gene expression and immunolocalization studies suggest that arabinan-containing pectins are potential in vivo substrates of ARAF1 in Arabidopsis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|