Protein size resolution in human eye lenses by dynamic light scattering after in vivo measurements
| Autor: | Robert Schwartz, Alfred Wegener, Matthias Dieckmann, Dirk Niederstrasser, Karsten Dierks |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Adult
Aging Adolescent Light genetic structures Scheimpflug principle Light scattering law.invention Lens protein Cellular and Molecular Neuroscience Optics Dynamic light scattering law Lens Crystalline medicine Animals Humans Scattering Radiation Child Aged Aged 80 and over Viscosity business.industry Scattering Spectrum Analysis Middle Aged Crystallins eye diseases Sensory Systems Optical axis Lens (optics) Ophthalmology medicine.anatomical_structure Regression Analysis Cattle Human eye sense organs business Densitometry |
| Zdroj: | Graefe’s Archive for Clinical and Experimental Ophthalmology. 236:18-23 |
| ISSN: | 1435-702X 0721-832X |
| Popis: | Dynamic light scattering (DLS) measurements were conducted with lowest intensity levels on human eye lenses (3.2 mW/cm2) within measurement times of 3-5 s. A characterisation of the human eye under in-vivo conditions along the optical axis is given and a careful interpretation of the data is made, referring not only to in-vitro results of investigated solutions of lens chemistry and various crystallin fractions but also to measurements performed on intact human lenses under various scattering angles. The clinical study was expanded to 79 subjects with ages varying from 9 to 85 years with no serious diseases of the ocular lenses. A normalisation of Scheimpflug photography density data to the data obtained by DLS enables comparison of the two techniques and shows good agreement. The bimodal character of the viscoelastic properties of healthy eye lenses was confirmed; with an assumed viscosity of 2 cP, the mean size parameter of the smaller component is 5.13 +/- 1.6 nm and of the polymeric fraction 690 nm. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink