B-Lymphocyte Proliferation during Bovine Leukemia VirusInduced Persistent Lymphocytosis Is Enhanced by T-Lymphocyte-Derived Interleukin-2
| Autor: | Esther S. Trueblood, Diana M. Stone, Wendy C. Brown, William C. Davis, Guy H. Palmer, Terry F. McElwain |
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| Rok vydání: | 1998 |
| Předmět: |
Interleukin 2
Lymphocytosis T-Lymphocytes medicine.medical_treatment Receptor expression Immunology Gene Expression Viral Pathogenesis and Immunity Biology Microbiology Peripheral blood mononuclear cell Virology Concanavalin A Leukemia Virus Bovine medicine Animals Humans RNA Messenger Antigens Viral Cells Cultured B-Lymphocytes Bovine leukemia virus Viral Core Proteins Receptors Interleukin-2 T lymphocyte biology.organism_classification Molecular biology Recombinant Proteins Interleukin-10 Kinetics Interleukin 10 Cytokine Culture Media Conditioned Insect Science Leukocytes Mononuclear Interleukin-2 Cattle Mitogens medicine.symptom Cell Division medicine.drug |
| Zdroj: | Journal of Virology. 72:3169-3177 |
| ISSN: | 1098-5514 0022-538X |
| Popis: | Bovine leukemia virus (BLV)-induced persistent lymphocytosis is characterized by a polyclonal expansion of CD5 + B lymphocytes. To examine the role of the cytokine microenvironment in this virus-induced B-lymphocyte expansion, the expression of interleukin-2 (IL-2), IL-4, IL-10, and gamma interferon (IFN-γ) mRNA, was measured in stimulated peripheral blood mononuclear cells from persistently lymphocytotic BLV-infected cows, nonlymphocytotic BLV-infected cows, and uninfected cows. IL-2 and IL-10 mRNA expression and IL-2 functional activity were significantly increased when peripheral blood mononuclear cells from persistently lymphocytotic cows were stimulated with concanavalin A (ConA). Additionally, during persistent lymphocytosis, peak IL-2 and IL-10 mRNA expression was delayed, and elevated expression was prolonged. To determine the potential biologic importance of increased IL-2 and IL-10 expression, the response of isolated B lymphocytes from persistently lymphocytotic cows to human recombinant cytokines and to cytokine-containing supernatants from isolated T lymphocytes was examined. While recombinant human IL-10 (rhIL-10) did not consistently induce detectable changes, rhIL-2 increased viral protein (p24) and IL-2 receptor expression in isolated B lymphocytes from persistently lymphocytotic cows. Additionally, rhIL-2 and supernatant from ConA-stimulated T lymphocytes enhanced B-lymphocyte proliferation. The stimulatory activity of the T-lymphocyte supernatant could be completely inhibited with a polyclonal anti-rhIL-2 antibody. Finally, polyclonal anti-rhIL-2 antibody, as well as anti-BLV antibody, inhibited spontaneous proliferation of peripheral blood mononuclear cells from persistently lymphocytotic cows, demonstrating that the spontaneous lymphoproliferation characteristic of BLV-induced persistent lymphocytosis is IL-2 dependent and antigen dependent. Collectively, these findings strongly suggest that increased T-lymphocyte expression of IL-2 in BLV-infected cows contributes to development and/or maintenance of persistent B lymphocytosis. |
| Databáze: | OpenAIRE |
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