Cardiac Transcription Factors Driven Lineage-Specification of Adult Stem Cells

Autor: José Anastasio Montero, José Manuel García-Verdugo, Elisa Lledó, Ana Armiñán, Carolina Gandía, José Luis Mullor, Pilar Sepúlveda
Rok vydání: 2010
Předmět:
Adult
Cellular differentiation
Active Transport
Cell Nucleus
Pharmaceutical Science
Biology
Sarcomerogenesis
Cardiac Transcription Factors
Neonatal Rat Cardiomyocytes
Genetics
Animals
Humans
Cell Lineage
Myocytes
Cardiac
Cells
Cultured
Genetics (clinical)
Body Patterning
Stem cell transplantation for articular cartilage repair
Cardiac Specification
Homeodomain Proteins
Myosin Heavy Chains
Troponin I
Mesenchymal stem cell
Cell Differentiation
Amniotic stem cells
Mesenchymal Stem Cells
Embryonic stem cell
Actins
Coculture Techniques
GATA4 Transcription Factor
Rats
Cell biology
Adult Stem Cells
Kinetics
Animals
Newborn
Immunology
Homeobox Protein Nkx-2.5
cardiovascular system
Molecular Medicine
Stem cell
Cardiology and Cardiovascular Medicine
Cardiac Myosins
Atrial Natriuretic Factor
Biomarkers
Transcription Factors
Adult stem cell
Zdroj: Journal of Cardiovascular Translational Research
r-CIPF: Repositorio Institucional Producción Científica del Centro de Investigación Principe Felipe (CIPF)
Centro de Investigación Principe Felipe (CIPF)
r-CIPF. Repositorio Institucional Producción Científica del Centro de Investigación Principe Felipe (CIPF)
instname
ISSN: 1937-5387
Popis: Differentiation of human bone marrow mesenchymal stem cells (hBMSC) into the cardiac lineage has been assayed using different approaches such as coculture with cardiac or embryonic cells, treatment with factors, or by seeding cells in organotypic cultures. In most cases, differentiation was evaluated in terms of expression of cardiac-specific markers at protein or molecular level, electrophysiological properties, and formation of sarcomers in differentiated cells. As observed in embryonic stem cells and cardiac progenitors, differentiation of MSC towards the cardiac lineage was preceded by translocation of NKX2.5 and GATA4 transcription factors to the nucleus. Here, we induce differentiation of hBMSC towards the cardiac lineage using coculture with neonatal rat cardiomyocytes. Although important ultrastructural changes occurred during the course of differentiation, sarcomerogenesis was not fully achieved even after long periods of time. Nevertheless, we show that the main cardiac markers, NKX2.5 and GATA4, translocate to the nucleus in a process characteristic of cardiac specification.
Databáze: OpenAIRE
Externí odkaz: