Preparation of 3H-labelled bongkrekate
Autor: | Wilfried Babel, K. Beyer, Heinrich Aquila, Martin Klingenberg |
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Jazyk: | angličtina |
Předmět: |
Magnetic Resonance Spectroscopy
Biophysics Mitochondria Liver Biology Tritium Biochemistry chemistry.chemical_compound Biosynthesis Structural Biology Genetics Methods Animals Bongkrekic Acid Molecular Biology Cell Biology Anti-Bacterial Agents Rats Kinetics Membrane chemistry Isotope Labeling Chromatography Thin Layer Bongkrekate Function (biology) |
Zdroj: | FEBS Letters. (2):124-127 |
ISSN: | 0014-5793 |
DOI: | 10.1016/0014-5793(76)81018-6 |
Popis: | 1. Introduction Bongkrekic acid (BKA) has turned out to be an extremely useful tool for research on the ADP, ATP carrier function [l-3] . In particular in the research on the binding to the ADP, ATP carrier sites in the membrane, bongkrekate was instrumental in elucidat- ing the reorientation mechanism for the translocator function [4-71. These studies were based on using radioactively labelled ADP and ATP in the 3H- or 14C-labelled form and ATR and CAT in the 35S- labelled form. It was therefore highly desirable, also to obtain radioactively labelled BKA*. Attempts to produce radioactively labelled BKA by biosynthesis analogous to ATR, had no success in producing BKA in yields sufficient for systematic binding studies (Klingenberg, unpublished) [8]. For this purpose the BKA producing organism |
Databáze: | OpenAIRE |
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