Preparation of 3H-labelled bongkrekate

Autor: Wilfried Babel, K. Beyer, Heinrich Aquila, Martin Klingenberg
Jazyk: angličtina
Předmět:
Zdroj: FEBS Letters. (2):124-127
ISSN: 0014-5793
DOI: 10.1016/0014-5793(76)81018-6
Popis: 1. Introduction Bongkrekic acid (BKA) has turned out to be an extremely useful tool for research on the ADP, ATP carrier function [l-3] . In particular in the research on the binding to the ADP, ATP carrier sites in the membrane, bongkrekate was instrumental in elucidat- ing the reorientation mechanism for the translocator function [4-71. These studies were based on using radioactively labelled ADP and ATP in the 3H- or 14C-labelled form and ATR and CAT in the 35S- labelled form. It was therefore highly desirable, also to obtain radioactively labelled BKA*. Attempts to produce radioactively labelled BKA by biosynthesis analogous to ATR, had no success in producing BKA in yields sufficient for systematic binding studies (Klingenberg, unpublished) [8]. For this purpose the BKA producing organism
Databáze: OpenAIRE