Selective cyclooxygenase-2 inhibition does not affect the healing of cutaneous full-thickness incisional wounds in SKH-1 mice
| Autor: | S H Kim, J J Casler, Medora M. Hardy, Eric A.G. Blomme, Catherine S. Tripp, K N Khan, Kevin S. Chinn, D Trajkovic, W A Hall, A C Opsahl |
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| Rok vydání: | 2003 |
| Předmět: |
Keratinocytes
Pathology medicine.medical_specialty Angiogenesis Blotting Western Anti-Inflammatory Agents Mice Nude Inflammation Mice Inbred Strains Dermatology Pharmacology Dexamethasone Mice medicine Animals Cyclooxygenase Inhibitors In Situ Hybridization Skin Wound Healing integumentary system biology Cyclooxygenase 2 Inhibitors business.industry Granulation tissue Precipitin Tests Isoenzymes medicine.anatomical_structure Cyclooxygenase 2 Prostaglandin-Endoperoxide Synthases biology.protein Celecoxib Female Cyclooxygenase medicine.symptom Keratinocyte Wound healing business Cell Division medicine.drug |
| Zdroj: | The British journal of dermatology. 148(2) |
| ISSN: | 0007-0963 |
| Popis: | SummaryBackground The inducible cyclooxygenase-2 (COX-2) enzyme is upregulated in inflammatory diseases, as well as in epithelial cancers, and has an established role in angiogenesis and tissue repair. Objective Because of these physiological effects and the widespread use of the selective COX-2 inhibitor, celecoxib, we wanted to determine if inhibition of COX-2 would affect incisional skin wound healing. Methods Using a cutaneous full-thickness, sutured, incisional wound model in hairless SKH-1 mice, we evaluated the role of COX-2 in the wound healing process by comparing the effects of a nonselective COX inhibitor, diclofenac, with a selective COX-2 inhibitor, SC-791. Healing was monitored for up to 28 days postincision histologically and for recovery of wound strength. Results COX-2 expression was observed over the first week of healing, peaking at day 3 and was not affected by treatment with the selective COX-2 or nonselective COX inhibitors. Infiltrating macrophages, as well as keratinocytes and dermal fibroblasts at the wound site, expressed COX-2. Neither selective COX-2, nor nonselective COX inhibition had a significant effect on the macroscopic or microscopic morphology of the wounds, whereas dexamethasone treatment resulted in epidermal and granulation tissue atrophy. In addition, neither selective COX-2, nor nonselective COX inhibition altered keratinocyte proliferation and differentiation, dermal angiogenesis or the recovery of wound tensile strength, whereas dexamethasone reduced the tensile strength of the wounds by 30–38% throughout the healing period. Conclusions These data indicate that selective COX-2 inhibition does not affect the healing of surgical skin wounds. |
| Databáze: | OpenAIRE |
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