Proton nuclear magnetic resonance spectroscopy of urine for rapid multicomponent analysis of intraoperative cellular metabolites
| Autor: | Shoichi Ohkochi, Yasuhide Iwao, Tsuneo Tatara, Junzo Takeda, Hisashi Uedaira, Yoshimasa Ishihara |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Adult
Male Surgical stress Metabolite Urinary system Clinical Biochemistry Ketone Bodies Urine Biochemistry chemistry.chemical_compound Nuclear magnetic resonance Glycosuria Stress Physiological Monitoring Intraoperative Humans Lactic Acid Amino Acids Nuclear Magnetic Resonance Biomolecular Biochemistry (medical) General Medicine Metabolism Perioperative Middle Aged chemistry Surgical Procedures Operative Ketone bodies Proton NMR Female Protons |
| Zdroj: | Clinica Chimica Acta. 279:117-124 |
| ISSN: | 0009-8981 |
| DOI: | 10.1016/s0009-8981(98)00174-0 |
| Popis: | We serially measured the proton nuclear magnetic resonance spectra of the urine of intraoperative patients over time to assess their potential use for rapid multicomponent analysis of cellular metabolites. Within a few minutes, the spectra provided signals of many low molecular weight urinary metabolites, including amino acids, ketone bodies, lactate, and glucose. The proton magnetic resonance spectra of the urine of most of the intraoperative patients showed large increases in urinary excretion of alanine, ketone bodies, and lactate and/or glucose, confirming alterations in energy substrate-endocrine relationships during the perioperative period. These metabolic changes appeared to be roughly proportional to the degree of surgical stress, but they were not consistent among patients who underwent the same operation. The study suggests that routine intraoperative metabolic monitoring is necessary to prevent critical metabolic disorders and that proton nuclear magnetic resonance spectroscopy of urine may meet this need by allowing rapid multicomponent analysis of cellular metabolites. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect