The arcA gene contributes to the serum resistance and virulence of Haemophilus parasuis serovar 13 clinical strain EP3
| Autor: | Yongping Wen, Xuefeng Yan, Yiping Wen, Xintian Wen, Lvqin He, Xiaobo Huang, Sanjie Cao, Rui Wu, Lingqiang Ding |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Serotype Haemophilus Infections Swine 030106 microbiology Virulence Serogroup Microbiology Pathogenesis Haemophilus parasuis Mice 03 medical and health sciences Bacterial Proteins Haemophilus Animals Serum Bactericidal Test Gene Sequence Deletion Swine Diseases Genetics Mice Inbred BALB C General Veterinary Strain (chemistry) biology Biofilm General Medicine biology.organism_classification Specific Pathogen-Free Organisms 030104 developmental biology Biofilms Female lipids (amino acids peptides and proteins) Anaerobic exercise Transcription Factors |
| Zdroj: | Veterinary Microbiology. 196:67-71 |
| ISSN: | 0378-1135 |
| DOI: | 10.1016/j.vetmic.2016.10.011 |
| Popis: | As a global transcriptional factor, ArcA regulates the expression of hundreds of genes involved in aerobic and anaerobic metabolism. Here we deleted arcA gene and investigated the biological characteristics of arcA deletion mutant (ΔarcA) in Haemophilus parasuis (H. parasuis) serovar 13 clinical strain EP3. Results indicated that deletion of arcA impaired growth of EP3 strain under anaerobic condition, and reduced virulence of EP3 strain in mice. Additionally, the ΔarcA strain showed greater sensitivity in porcine serum and produced less biofilm mass than the EP3 strain. Taken together, these findings suggested that the arcA gene may be involved in pathogenesis in Haemophilus parasuis. |
| Databáze: | OpenAIRE |
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