Coexpression Analysis Identified PcMYB25 as a Patchoulol Synthase Gene Activator to Enhance Patchouli Alcohol Biosynthesis in Pogostemon Cablin

Autor: Baiyang Zhong, Xuanxuan Zhou, Xilin Wang, Xiaobing Wang, Ruoting Zhan, Junren Li, Huiling Huang, Daidi Wu, Likai Chen, Xiuchen Chen
Rok vydání: 2021
Předmět:
DOI: 10.21203/rs.3.rs-207385/v2
Popis: Background Patchouli alcohol is an effective component of the medicinal plant patchouli. Similar to other secondary metabolites, its synthesis is also regulated by transcription factors. Although the biosynthetic pathway of patchouli alcohol has been characterized, the regulatory mechanism of patchouli alcohol has not been fully revealed. Results This study combined the transcriptome data of patchouli leaves treated with different hormones and WGCNA to complete the coexpression network. The modules related to patchouli alcohol were identified, and PcMYB25 played a crucial role in regulating patchouli alcohol biosynthesis. The overexpression of PcMYB25 can promote the expression of PTS , thereby increasing the content of patchouli alcohol. Conclusions This is the first reporter that MYB25 regulates the secondary metabolism of patchouli. These experimental results lay the foundation for further analysis of the regulatory mechanism of patchouli alcohol synthesis.
Databáze: OpenAIRE