Molecular Cloning, Structure and Phylogenetic Analysis of a Hemocyanin Subunit from the Black Sea Crustacean Eriphia verrucosa (Crustacea, Malacostraca)
| Autor: | Martin Ivanov, Pavlina Dolashka, Alexandar Dolashki, Mariana Radkova, Elena Todorovska |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Signal peptide lcsh:QH426-470 glycosylation Protein subunit medicine.medical_treatment Article Evolution Molecular 03 medical and health sciences 0302 clinical medicine Decapoda Complementary DNA Crustacea Hemolymph Genetics medicine Animals structure Cloning Molecular Genetics (clinical) Eriphia verrucosa Phylogeny chemistry.chemical_classification biology phylogenetic analysis Brachyura) Hemocyanin Eriphia verrucosa (Crustacea 3-D model biology.organism_classification Amino acid lcsh:Genetics Open reading frame 030104 developmental biology chemistry Biochemistry Black Sea 030220 oncology & carcinogenesis Hemocyanins cDNA cloning hemocyanin |
| Zdroj: | Genes Volume 12 Issue 1 Genes, Vol 12, Iss 93, p 93 (2021) |
| ISSN: | 2073-4425 |
| Popis: | Hemocyanins are copper-binding proteins that play a crucial role in the physiological processes in crustaceans. In this study, the cDNA encoding hemocyanin subunit 5 from the Black sea crab Eriphia verrucosa (EvHc5) was cloned using EST analysis, RT-PCR and rapid amplification of the cDNA ends (RACE) approach. The full-length cDNA of EvHc5 was 2254 bp, consisting of a 5&prime and 3&prime untranslated regions and an open reading frame of 2022 bp, encoding a protein consisting of 674 amino acid residues. The protein has an N-terminal signal peptide of 14 amino acids as is expected for proteins synthesized in hepatopancreas tubule cells and secreted into the hemolymph. The 3D model showed the presence of three functional domains and six conserved histidine residues that participate in the formation of the copper active site in Domain 2. The EvHc5 is O-glycosylated and the glycan is exposed on the surface of the subunit similar to Panulirus interruptus. The phylogenetic analysis has shown its close grouping with &gamma type of hemocyanins of other crustacean species belonging to order Decapoda, infraorder Brachyura. |
| Databáze: | OpenAIRE |
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