Molecular Parameters Involved in the Estrogenicity of Mestranol and Ethynylestradiol
| Autor: | D W, Hahn, J L, McGuire, F C, Greenslade, G D, Turner, J P, DaVanzo |
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| Rok vydání: | 1971 |
| Předmět: |
medicine.medical_specialty
Population Uterus Estrogen receptor Pharmacology Ethinyl Estradiol Tritium General Biochemistry Genetics and Molecular Biology Mice Internal medicine Centrifugation Density Gradient Methods medicine Animals Drug Interactions Castration education Receptor Uridine education.field_of_study Binding Sites Estradiol business.industry Ovary Mestranol RNA Chromatography Ion Exchange Endocrinology medicine.anatomical_structure Hormone receptor Ovariectomized rat Female Chromatography Thin Layer Rabbits business medicine.drug |
| Zdroj: | Experimental Biology and Medicine. 137:1180-1185 |
| ISSN: | 1535-3699 1535-3702 |
| Popis: | 2 molecular parameters of estrogenicity binding to estrogen receptors and induction of uterine RNA synthesis were used to compare the estrogenicity of mestranol and ethynylestradiol. White rabbits and immature mice were used in cytosol incubation studies while white female ovariectomized rats were used in sucrose density gradient isolation experiments. Ovariectomized female mice were used in the RNA synthesis studies. Uteri of the animals were examined after injection of the compound being tested. Following injection of mestranol both mestranol and ethynylestradiol accumulated in the uterus with a predominence of ethynylestradiol. Through experimentation it was shown that ethynylestradiol has a greater binding affinity for its receptor than does mestranol. At doses of .1 mcg/animal only ethynylestradiol induced significant RNA synthesis. Only at a markedly elevated dose did mestranol generate equivalent RNA synthesis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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