Optimization of growth media for obtaining high-cell density cultures of halophilic archaea (family Halobacteriaceae) by response surface methodology
| Autor: | Muthu Manikandan, Lejla Pašić, Vijayaraghavan Kannan |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Quality Control
Environmental Engineering Starch Cell Culture Techniques Artificial seawater Biomass Cell Count Bioengineering Models Biological Microbiology chemistry.chemical_compound Bioreactors Halobacterium salinarum Halobacteriaceae Computer Simulation Food science Response surface methodology Waste Management and Disposal Cell Proliferation Halobacteriales biology Renewable Energy Sustainability and the Environment General Medicine biology.organism_classification Halophile Culture Media chemistry Algorithms |
| Zdroj: | Bioresource Technology. 100:3107-3112 |
| ISSN: | 0960-8524 |
| DOI: | 10.1016/j.biortech.2009.01.033 |
| Popis: | Optimization of media components for the growth and biomass production of Halobacterium salinarum VKMM 013 was carried out using response surface methodology. A second order quadratic model was estimated and media components were determined based on quadratic regression equation generated by model. These were 6.35 g L(-1) of KCl, 9.70 g L(-1) of MgSO(4), 13.38 g L(-1) of gelatin and 12.00 g L(-1) of soluble starch in nutrient broth supplemented with artificial seawater with 20% (w/v) of NaCl. In these optimal conditions, the obtained cell concentration of 0.746 g L(-1) dry weight was in agreement with the predicted cell concentration. The optimized media significantly shortened the time required for cell culture to reach the stationary phase while providing a nearly 2.4-fold increase in biomass production. Furthermore, in cell cultures of three other halophilic archaea the use of optimized media enhanced growth rate and provided high-cell density. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect