Expression of simian CETP in normolipidemic Fisher rats has a profound effect on large sized apoE-containing HDL
| Autor: | Gillian Brooker, Jean-Paul Pais de Barros, Delphine Autran, Alain Bataillard, Valérie Deckert, Laure Dumont, Zoulika Zak, Martina Schneider, David Masson, Laurent Lagrost, Valérie Risson, Linda Duverneuil, Philippe Moulin, Thomas Gautier, Naig Le Guern, Jean Sassard |
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| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: |
Apolipoprotein E
Male medicine.medical_specialty Heterozygote QD415-436 Simian transgenesis Biochemistry Animals Genetically Modified chemistry.chemical_compound Endocrinology Apolipoproteins E In vivo Internal medicine Total cholesterol Cholesterylester transfer protein medicine Animals RNA Messenger CETP deficiency Glycoproteins apolipoprotein E biology Chemistry Cholesterol Cholesterol HDL Heterozygote advantage Cell Biology biology.organism_classification Rats Inbred F344 Cholesterol Ester Transfer Proteins Rats carbohydrates (lipids) High plasma biology.protein Female lipids (amino acids peptides and proteins) Carrier Proteins |
| Zdroj: | Journal of Lipid Research, Vol 43, Iss 12, Pp 2164-2171 (2002) |
| ISSN: | 0022-2275 |
| Popis: | In order to investigate the direct effect of cholesteryl ester transfer protein (CE22285) on the structure and composition of HDL in vivo, simian CETP was expressed in Fisher rat that spontaneously displays high plasma levels of HDL1. In the new CETPTg rat line, the production of active CETP by the liver induced a significant 48% decrease in plasma HDL cholesterol, resulting in a 34% decrease in total cholesterol level (P < 0.01 in both cases). Among the various plasma HDL subpopulations, the largest HDL were those mostly affected by CETP, with a 74% decrease in HDL1 versus a significantly weaker 38% decrease in smaller HDL2 (P < 0.0001). Apolipoprotein E (apoE)-containing HDL1 were selectively affected by CETP expression, whereas apoA content of HDL remained unmodified. The reduction in the apoE content of serum HDL observed in CETPTg rats compared to controls (53%, P < 0.02) suggests that apoE in HDL may constitute in vivo a major determinant of their ability to interact with CETP.These results bring new insight into the lack of HDL1 in plasma from CETP-deficient heterozygotes despite their substantial 50% decrease in CETP activity. In addition, they indicate that HDL1 constitute reliable and practicable sensors of very low plasma CETP activity in vivo. |
| Databáze: | OpenAIRE |
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