Antiproliferative and apoptotic effects of probiotic whey dairy beverages in human prostate cell lines
| Autor: | Adriano G. Cruz, Marcia C. Silva, Erick A. Esmerino, Mônica Q. Freitas, Lana de Souza Rosa, Anderson Junger Teodoro, Mariana L. Santos, Maria Carmela K.H. Duarte, Joel Pimentel de Abreu, Tatiana Colombo Pimentel, Hugo L.A. Silva, Ramon S. Rocha, Celso F. Balthazar |
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| Rok vydání: | 2020 |
| Předmět: |
Male
030309 nutrition & dietetics Cell Line law.invention Flow cytometry Beverages 03 medical and health sciences Probiotic fluids and secretions 0404 agricultural biotechnology Lactobacillus acidophilus law Whey Lactobacillus medicine Humans MTT assay Viability assay Food science 0303 health sciences biology medicine.diagnostic_test Chemistry Probiotics digestive oral and skin physiology Prostate food and beverages 04 agricultural and veterinary sciences biology.organism_classification 040401 food science Bifidobacterium animalis Apoptosis Food Science |
| Zdroj: | Food Research International. 137:109450 |
| ISSN: | 0963-9969 |
| DOI: | 10.1016/j.foodres.2020.109450 |
| Popis: | The present study aimed to evaluate the antiproliferative and apoptotic effects of probiotic whey dairy beverages in human prostate cancer cell lines (PC-3 and DU-145). Five different whey beverages were manufactured: conventional whey beverage (without addition of probiotic strain, CTL); and whey beverages containing Lactobacillus acidophilus La-05, Lactobacillus acidophilus La-03, Lactobacillus casei-01, and Bifidobacterium animalis Bb-12. Cell viability was determined by MTT assay and cell cycle arrest, and apoptosis by flow cytometry. All the samples presented cytotoxic activities against both cell lines. A decrease in the percentage of PC-3 cells in G0/G1 and S, followed by an increase in G2/M phase were observed with L. casei-01, Bb-12 and La-05 beverages (50.0 and 100.0 µg/mL). The extracts of the whey beverages caused extensive apoptosis induction in both cells’ lines, regardless of the probiotic strain. However, the whey beverage added with L. casei-01 might be a better candidate against prostate cancer cells. |
| Databáze: | OpenAIRE |
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