Cellular Import Mediated by Nuclear Localization Signal Peptide Sequences
| Autor: | Angela D. Ragin, Jean Chmielewski, Rashida A Morgan |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Nuclear Localization Signals
Clinical Biochemistry Oligonucleotides Peptide Biology Biochemistry Flow cytometry law.invention Drug Delivery Systems Confocal microscopy law Drug Discovery Tumor Cells Cultured medicine Humans NLS Molecular Biology Fluorescent Dyes chemistry.chemical_classification Pharmacology Microscopy Confocal medicine.diagnostic_test Oligonucleotide Rational design General Medicine Flow Cytometry Cell Compartmentation Cell biology chemistry Cytoplasm Molecular Medicine Nuclear localization sequence Transcription Factors |
| Zdroj: | Chemistry & Biology. 9(8):943-948 |
| ISSN: | 1074-5521 |
| DOI: | 10.1016/s1074-5521(02)00189-8 |
| Popis: | The cellular delivery of therapeutic agents and their localization within cells is currently a great challenge in medicinal chemistry. A few cationic peptides have shown a strong propensity to cross the cytoplasmic membrane and enter cells. Nuclear localization signal (NLS) sequences are a class of highly cationic peptides that may be exploited for cellular import of linked cargo. A series of NLS sequence peptides were investigated for entry into different cancer cell lines by flow cytometry and confocal microscopy. All NLS peptides demonstrated rapid accumulation within cells when added to the cellular media. Covalent adducts of proteins and oligonucleotides with NLS peptides were also effectively imported within cells. An understanding of the structural and mechanistic properties of these sequences will provide great potential for the rational design of efficient and selective peptidic delivery systems. |
| Databáze: | OpenAIRE |
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