Suckling-induced Fos activation and melanin-concentrating hormone immunoreactivity during late lactation
Autor: | Jackson C. Bittencourt, Luciano Freitas Felício, Renato Duarte Alvisi, J.M. Da-Silva, Giovanne B. Diniz |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
medicine.medical_specialty Melanin-concentrating hormone Hypothalamus Melanophores Stimulation Biology General Biochemistry Genetics and Molecular Biology Energy homeostasis 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Lactation Internal medicine medicine Weaning Animals General Pharmacology Toxicology and Pharmaceutics Rats Wistar Melanins Sensory stimulation therapy Hypothalamic Hormones Colocalization HORMÔNIO CONCENTRADOR DE MELANINA General Medicine Animals Suckling Rats Pituitary Hormones 030104 developmental biology Endocrinology medicine.anatomical_structure chemistry Female Proto-Oncogene Proteins c-fos hormones hormone substitutes and hormone antagonists 030217 neurology & neurosurgery Hormone |
Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
Popis: | Aims Melanin-concentrating hormone (MCH) is implicated in the control of food intake, body weight regulation and energy homeostasis. Lactation is an important physiological model to study the hypothalamic integration of peripheral sensory signals, such as suckling stimuli and those related to energy balance. MCH can be detected in the medial preoptic area (MPOA), especially around the 19th day of lactation, when this hormone is described as displaying a peak synthesis followed by a decrease after weaning. The physiological significance of this phenomenon is unclear. Therefore, we aimed to investigate hypothalamic changes associated to sensory stimulation by the litter, in special its influence over MCH synthesis. Main methods Female Wistar rats (n = 56) were euthanized everyday from lactation days 15–21, with or without suckling stimulus (WS and NS groups, respectively). MCH and Fos immunoreactivity were evaluated in the MPOA and lateral and incerto-hypothalamic areas (LHA and IHy). Key findings Suckling stimulus induced Fos synthesis in all regions studied. An increase on the number of suckling-induced Fos-ir neurons could be detected in the LHA after the 18th day. Conversely, the amount of MCH decreased in the MPOA from days 15–21, independent of suckling stimulation. No colocalization between MCH and Fos could be detected in any region analyzed. Significance Suckling stimulus is capable of stimulating hypothalamic regions not linked to maternal behavior, possibly to mediate energy balance aspects of lactation. Although dams are hyperphagic before weaning, this behavioral change does not appear to be mediated by MCH. |
Databáze: | OpenAIRE |
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