Acute toxicity of textile dye Methylene blue on growth and metabolism of selected freshwater microalgae
| Autor: | Abarna Krishna Moorthy, Satya Prakash Shukla, Kundan Kumar, Bhuvaneswari Govindarajan Rathi, V. S. Bharti |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Chlorophyll
Health Toxicology and Mutagenesis Chlorella vulgaris Industrial Waste Fresh Water 010501 environmental sciences Toxicology 01 natural sciences 03 medical and health sciences Pigment chemistry.chemical_compound Microalgae Spirulina Food science Coloring Agents Carotenoid Plant Proteins 030304 developmental biology 0105 earth and related environmental sciences EC50 Pharmacology chemistry.chemical_classification Spirulina (genus) 0303 health sciences biology Textiles General Medicine biology.organism_classification Carotenoids Acute toxicity Methylene Blue chemistry visual_art Toxicity visual_art.visual_art_medium Water Pollutants Chemical Methylene blue |
| Zdroj: | Environmental Toxicology and Pharmacology. 82:103552 |
| ISSN: | 1382-6689 |
| Popis: | Microalgae are ecologically important species in aquatic ecosystems due to their role as primary producers. The inhibition of growth of microalgae due to dye pollution results in an upheaval in the trophic transfer of nutrients and energy in aquatic ecosystems. Therefore, this investigation aimed to evaluate the toxicity of a textile dye Methylene blue (MB) on two microalgae viz. Chlorella vulgaris and Spirulina platensis. An exposure of the unialgal populations of both the microalgae towards graded concentrations of the dye showed a concentration-dependent decrease in specific growth rate, pigment and protein content. In the toxicity study of 24 -96-h, following the OECD guidelines 201, the EC50 values of C. vulgaris and S. platensis ranged from 61.81 to 5.43 mg/L and 5.83 to 1.08 mg/L respectively revealing that S. platensis exhibited a higher level of susceptibility towards the dye as compared to C. vulgaris and the latter is more tolerant to the dye toxicity even at higher concentrations. The findings indicate that the response to dye is a species-specific phenomenon. Given the differences in the cell structure and enzymatic pathways in Spirulina platensis (a prokaryote) and Chlorella vulgaris (an eukaryote), the tolerance levels can differ. After 96-h exposure of C. vulgaris to MB (100 mg/L), the chlorophyll-a, b and carotenoid content were reduced 2.5, 5.96 and 3.57 times in comparison to control whereas in S. platensis exposure to MB (10 mg/L), the chlorophyll-a and carotenoid content were reduced 3.59 and 5.08 times in comparison to control. After 96-h exposure of C. vulgaris and S. platensis to the dye (20 mg/L), the protein content was found to be 4.34 and 2.75 times lower than the control. The protein content has decreased in accordance with the increase in dye concentration. |
| Databáze: | OpenAIRE |
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