Curcumin modulates endothelial permeability and monocyte transendothelial migration by affecting endothelial cell dynamics
Autor: | Radu Tamaian, Nicolas Barber-Chamoux, Christine Morand, Dragan Milenkovic, Laurent-Emmanuel Monfoulet, Dominique Bayle, Sylvie Mercier |
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Přispěvatelé: | Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Research and Development Department, National Institute for Research and Development for Cryogenic and Isotopic Technologies [Valcea] (ICSI), SC Biotech Corp SRL, Service de Cardiologie [CHU de Dijon], Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), COST Action FA1403-POSITIVe, Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), Service de Cardiologie |
Jazyk: | angličtina |
Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
transendothelial migration migration cellulaire THP-1 Cells [SDV]Life Sciences [q-bio] Biochemistry Umbilical vein chemistry.chemical_compound Cell Movement curcumin Cytoskeleton Kinase Chemistry NF-kappa B MAP Kinase Kinase Kinases Biomechanical Phenomena Cell biology Molecular Docking Simulation Endothelial stem cell medicine.anatomical_structure endothelial cell Diffusion Chambers Culture endothelial permeability Tumor necrosis factor alpha Signal transduction Rheology Signal Transduction monocyte adhesion perméabilité Protein Serine-Threonine Kinases 03 medical and health sciences Physiology (medical) Cell Adhesion Human Umbilical Vein Endothelial Cells medicine Humans Tumor Necrosis Factor-alpha Gene Expression Profiling Monocyte Pyruvate Dehydrogenase Acetyl-Transferring Kinase Microarray Analysis Coculture Techniques Cytoskeletal Proteins curcumine 030104 developmental biology Gene Expression Regulation Curcumin cell trafficking permeability Proto-Oncogene Proteins c-akt cellule endotheliale |
Zdroj: | Free Radical Biology and Medicine Free Radical Biology and Medicine, 2017, 112, pp.109-120. ⟨10.1016/j.freeradbiomed.2017.07.019⟩ Free Radical Biology and Medicine, Elsevier, 2017, 112, pp.109-120. ⟨10.1016/j.freeradbiomed.2017.07.019⟩ |
ISSN: | 0891-5849 |
Popis: | Curcumin is a phenolic compound that exhibits beneficial properties for cardiometabolic health. We previously showed that curcumin reduced the infiltration of immune cells into the vascular wall and prevented atherosclerosis development in mice. This study aimed to investigate the effect of curcumin on monocyte adhesion and transendothelial migration (TEM) and to decipher the underlying mechanisms of these actions. Human umbilical vein endothelial cells (HUVECs) were exposed to curcumin (0.5-1μM) for 3h prior to their activation by Tumor Necrosis Factor alpha (TNF-α). Endothelial permeability, monocyte adhesion and transendothelial migration assays were conducted under static condition and shear stress that mimics blood flow. We further investigated the impact of curcumin on signaling pathways and on the expression of genes using macroarrays. Pre-exposure of endothelial cells to curcumin reduced monocyte adhesion and their transendothelial migration in both static and shear stress conditions. Curcumin also prevented changes in both endothelial permeability and the area of HUVECs when induced by TNF-α. We showed that curcumin modulated the expression of 15 genes involved in the control of cytoskeleton and endothelial junction dynamic. Finally, we showed that curcumin inhibited NF-κB signaling likely through an antagonist interplay with several kinases as suggested by molecular docking analysis. Our findings demonstrate the ability of curcumin to reduce monocyte TEM through a multimodal regulation of the endothelial cell dynamics with a potential benefit on the vascular endothelial function barrier. |
Databáze: | OpenAIRE |
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